Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense
文献类型：期刊论文
| 作者 | Qu, Tao1; Zhang, Linlin1; Wang, Wei1; Huang, Baoyu1,2 ; Li, Yingxiang1,2; Zhu, Qihui1,2; Li, Li1; Zhang, Guofan1
|
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2015-07-01 |
| 卷号 | 51期号:1页码:74-78 |
| 关键词 | Crassostrea gigas IAP Evolution Intrinsic apoptosis Caspase Immune response |
| 英文摘要 | The inhibitor of apoptosis (IAP) proteins maintain a balance between cell proliferation and cell death by inhibiting caspase activity and facilitating immune responses. In this study, phylogenetic analysis revealed lineage-specific expansion and tandem duplication of IAPs in the Pacific oyster Crassostrea gigas. We then investigated a representative oyster-specific XIAP-like gene (CgIAP2) to understand how it regulates initiator caspase. Cloning of full-length CgIAP2 from oyster cDNA uncovered a deduced protein containing two BIR domains and a RING domain. Homolog comparison demonstrated that CgIAP2 clustered into the invertebrate branch. We found that CgIAP2 was likely involved in apoptosis inhibition and immune defense, based on high mRNA expression in the gills and labial palps, as well as increased mRNA expression after bacterial challenge. A yeast two-hybrid assay revealed that the BIR2 domain was necessary and sufficient to mediate interaction between CgIAP2 and Cgcaspase-2, providing direct evidence that CgIAP2 participates in apoptosis inhibition. (C) 2015 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000357906800009 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/49050]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qu, Tao,Zhang, Linlin,Wang, Wei,et al. Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):74-78. |
| APA | Qu, Tao.,Zhang, Linlin.,Wang, Wei.,Huang, Baoyu.,Li, Yingxiang.,...&Zhang, Guofan.(2015).Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(1),74-78. |
| MLA | Qu, Tao,et al."Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.1(2015):74-78. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。