Transcriptome sequencing and characterization for Kappaphycus alvarezii
文献类型：期刊论文
| 作者 | Zhang, Zhen1; Pang, Tong1; Li, Qianqian1,2 ; Zhang, Litao1; Li, Ling1; Liu, Jianguo1
|
| 刊名 | EUROPEAN JOURNAL OF PHYCOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 50期号:4页码:400-407 |
| 关键词 | functional annotation Kappaphycus alvarezii KEGG pathway Rhodophyta RPKM transcriptome |
| 英文摘要 | The transcriptome of Kappaphycus alvarezii was profiled using high-throughput Solexa paired-end sequencing technology. A total of 61 million sequencing reads was generated by filtering the low-quality reads, and 28 701 unigenes with a mean length of 901 bp were obtained based on de novo assembly. In similarity alignments against the NCBI non-redundant protein sequence (NR) database, Swissprot database, Cluster of Orthologous Groups (COG) database, Gene ontology (GO) database, and Kyoto Encyclopedia of Genes and Genomes (KEGG) database, 11 996 (41.79%) unigenes were identified with significant hits (E-value < 10(-5)) against existing genes. Functional annotation with the KEGG pathway database identified 8975 unigenes and mapped to 125 pathways. Through functional enrichment analysis of the genes with a higher expression value than the average RPKM (reads per kilobase per million reads), we found that some important pathways were highly expressed. The substantial number of transcript sequences of K. alvarezii provides a valuable resource for potential gene identification and comparative genomic studies. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000363951900004 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/82280]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zhen,Pang, Tong,Li, Qianqian,et al. Transcriptome sequencing and characterization for Kappaphycus alvarezii[J]. EUROPEAN JOURNAL OF PHYCOLOGY,2015,50(4):400-407. |
| APA | Zhang, Zhen,Pang, Tong,Li, Qianqian,Zhang, Litao,Li, Ling,&Liu, Jianguo.(2015).Transcriptome sequencing and characterization for Kappaphycus alvarezii.EUROPEAN JOURNAL OF PHYCOLOGY,50(4),400-407. |
| MLA | Zhang, Zhen,et al."Transcriptome sequencing and characterization for Kappaphycus alvarezii".EUROPEAN JOURNAL OF PHYCOLOGY 50.4(2015):400-407. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。