Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus
文献类型：期刊论文
| 作者 | Zheng, Y.1; Peng, L. M.1,2; You, F.1; Zou, Y. X.1; Zhang, P. J.1; Chen, S. L.3 |
| 刊名 | JOURNAL OF FISH BIOLOGY
 |
| 出版日期 | 2015-07-01 |
| 卷号 | 87期号:1页码:115-122 |
| 关键词 | astrocyte brain-cell line epithelioid-like karyotype transfection |
| 英文摘要 | A new brain-cell line derived from Japanese flounder Paralichthys olivaceus (POBC) was established. POBC was subcultured for 67 passages over the course of 420 days. The cultured cells were primarily epithelioid-like. Chromosome analysis revealed the cell line to possess the normal P. olivaceus diploid karyotype of 2n = 48t (telocentric chromosomes). The cells exhibited the astrocyte marker glial fibrillary acidic protein by immunocytochemistry, and significant fluorescent signals were observed when the cells were transfected with green fluorescent protein reporter plasmid. The established POBC would be ideal material for the study of function of fish ependyma, the central neuroendocrine system and endocrine disruptors in the marine environment. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000357686800009 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/48988]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Y.,Peng, L. M.,You, F.,et al. Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus[J]. JOURNAL OF FISH BIOLOGY,2015,87(1):115-122. |
| APA | Zheng, Y.,Peng, L. M.,You, F.,Zou, Y. X.,Zhang, P. J.,&Chen, S. L..(2015).Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus.JOURNAL OF FISH BIOLOGY,87(1),115-122. |
| MLA | Zheng, Y.,et al."Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus".JOURNAL OF FISH BIOLOGY 87.1(2015):115-122. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。