Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Yang, Hui1,2 ; Li, Shihao1,3; Li, Fuhua1,3; Wen, Rong1; Xiang, Jianhai1
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| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 出版日期 | 2015-08-01 |
| 卷号 | 51期号:2页码:278-286 |
| 关键词 | Syndecan WSSV infection siRNA Penaeid shrimp |
| 英文摘要 | Syndecan is considered to be a multifunctional protein which functions as a cell surface receptor involved in cell adhesion, migration, cytoskeleton organization and differentiation. Previous bioinformatic analysis has revealed that syndecan in shrimp might interact with white spot syndrome virus (WSSV). In the present study, we experimentally studied the function of syndecan in shrimp immunity. The syndecan from Litopenaeus vannamei (LvSDC) was cloned and analyzed. The full-length cDNA of LvSDC was 1005 bp, consisting of 59 bp 5'-UTR, 253 bp 3'-UTR, and 693 bp open reading frame encoding 230 amino acids. LvSDC consisted of an extracellular domain (ED), a transmembrane domain (TM) and a cytoplasmic domain (CD). TM and CD shared high similarities with those of syndecan proteins from other species. LvSDC was ubiquitously expressed in all tested tissues, with the highest level in Oka. After WSSV challenge, the transcription level of LvSDC in Oka was apparently up-regulated. Recombinant LvSDC protein and its rabbit polyclonal antibody were prepared for detecting the location of LvSDC in hemocytes using immunocytochemistry approach. Data showed that LvSDC mainly located at the cell membrane and the cytoplasm of hemocytes. After silencing of LvSDC with siRNA, the WSSV copy numbers and mortality of shrimp after WSSV infection were both significantly decreased. These data provide useful information for understanding the immune mechanism of shrimp to WSSV infection. (C) 2015 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000357910600007 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/49049]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Hui,Li, Shihao,Li, Fuhua,et al. Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(2):278-286. |
| APA | Yang, Hui,Li, Shihao,Li, Fuhua,Wen, Rong,&Xiang, Jianhai.(2015).Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(2),278-286. |
| MLA | Yang, Hui,et al."Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.2(2015):278-286. |
入库方式： OAI收割
来源：海洋研究所
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