Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis
文献类型：期刊论文
| 作者 | Liu, Li1,2 ; Chi, Heng1; Sun, Li1
|
| 刊名 | DISEASES OF AQUATIC ORGANISMS
 |
| 出版日期 | 2015-06-29 |
| 卷号 | 115期号:1页码:67-80 |
| 关键词 | Pseudomonas fluorescens Ferric uptake regulator Proteomic Infection Virulence Vaccine |
| 英文摘要 | Pseudomonas fluorescens is a Gram-negative bacterium and a common pathogen to a wide range of farmed fish. In a previous study, we found that the ferric uptake regulator gene (fur) is essential to the infectivity of a pathogenic fish isolate of P. fluorescens (wild-type strain TSS). In the present work, we conducted comparative proteomic analysis to examine the global protein profiles of TSS and the P. fluorescens fur knockout mutant TFM. Twenty-eight differentially produced proteins were identified, which belong to different functional categories. Four of these proteins, viz. TssP (a type VI secretion protein), PspA (a serine protease), OprF (an outer membrane porin), and ClpP (the proteolytic subunit of an ATP-dependent Clp protease), were assessed for virulence participation in a model of turbot Scophthalmus maximus. The results showed that the oprF and clpP knockouts exhibited significantly reduced capacities in (1) resistance against the bactericidal effect of host serum, (2) dissemination into and colonization of host tissues, and (3) inducing host mortality. In contrast, mutation of tssP and pspA had no apparent effect on the pathogenicity of TSS. Purified recombinant OprF, when used as a subunit vaccine, induced production of specific serum antibodies in immunized fish and elicited significant protection against lethal TSS challenge. Antibody blocking of the OprF in TSS significantly impaired the ability of the bacteria to invade host tissues. Taken together, these results indicate for the first time that in pathogenic P. fluorescens, Fur regulates the expression of diverse proteins, some of which are required for optimal infection. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000357598200008 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/49204]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Li,Chi, Heng,Sun, Li. Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis[J]. DISEASES OF AQUATIC ORGANISMS,2015,115(1):67-80. |
| APA | Liu, Li,Chi, Heng,&Sun, Li.(2015).Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis.DISEASES OF AQUATIC ORGANISMS,115(1),67-80. |
| MLA | Liu, Li,et al."Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis".DISEASES OF AQUATIC ORGANISMS 115.1(2015):67-80. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。