Construction and characterization of the angR mutant of Vibrio anguillarum via insertional inactivation
文献类型：期刊论文
| 作者 | Zhang, Zhendong1; Mo, Zhaolan2; Chen, Shiyong2; Zou, YuXia2; Zhang, Peijun2 |
| 刊名 | JOURNAL OF ENVIRONMENTAL BIOLOGY
 |
| 出版日期 | 2015-07-01 |
| 卷号 | 36期号:2015页码:807-811 |
| 关键词 | angR gene Iron uptake system Insertional inactivation Vibrio anguillarum |
| 英文摘要 | Vibrio anguillarum strain M3 was isolated from cultured diseased flounder Paralichthys olivaceus. In order to determine whether chromosomal angR gene plays an important role in infecting flounder host, part fragment of a virulence regulatory gene angR from V anguillarum M3 genome was cloned and ligated with suicide plasmids pNQ705 in the present study. The conserved fragment of angR gene was amplified from M3 genome and inserted into suicide plasmid pNQ705, a kind of conditional replicon. The recombinant plasmid was transferred into Vibrio anguillarum M3 strain genomic DNA through bacterial conjugation and homologous recombination. A mutant V anguillarum strain with angR gene mutation was constructed and screened successfully using TCBS medium containing chloramphenicol. PCR identification and sequencing showed that the recombinant plasmid inserted into Vibrio anguillarum genome DNA, as expected design, resulted in insertional inactivation of angR gene. Results of artificial infection experiment showed that virulence of mutant Vibrio anguillarum strain reduced dramatically as compared with the wild strain M3. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000358754200014 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/49673]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Natl Marine Environm Monitoring Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inst Oceanog, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zhendong,Mo, Zhaolan,Chen, Shiyong,et al. Construction and characterization of the angR mutant of Vibrio anguillarum via insertional inactivation[J]. JOURNAL OF ENVIRONMENTAL BIOLOGY,2015,36(2015):807-811. |
| APA | Zhang, Zhendong,Mo, Zhaolan,Chen, Shiyong,Zou, YuXia,&Zhang, Peijun.(2015).Construction and characterization of the angR mutant of Vibrio anguillarum via insertional inactivation.JOURNAL OF ENVIRONMENTAL BIOLOGY,36(2015),807-811. |
| MLA | Zhang, Zhendong,et al."Construction and characterization of the angR mutant of Vibrio anguillarum via insertional inactivation".JOURNAL OF ENVIRONMENTAL BIOLOGY 36.2015(2015):807-811. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。