ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate
文献类型：期刊论文
| 作者 | Zhang, Jian1,2; Li, Mo-fei1
|
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2015-08-01 |
| 卷号 | 45期号:2页码:486-494 |
| 关键词 | Megalocytivirus DNA vaccine Scophthalmus maximus Immune response |
| 英文摘要 | Megalocytivirus, a DNA virus belonging to the Iridoviridae family, is a severe pathogen to a wide range of marine and freshwater fish. In this study, using turbot (Scophthalmus maximus) as a host model, we examined the immunoprotective property of one megalocytivirus gene, ORF75, in the form of DNA vaccine (named pORF75). Immunofluorescence microscopy and RT-PCR analysis showed that P444, the protein encoded by ORF75, was naturally produced in the tissues of turbot during megalocytivirus infection, and that the vaccine gene in pORF75 was expressed in fish cells transfected with pORF75 and in the tissues of turbot immunized with pORF75. Following vaccination of turbot with pORF75, a high level of survival (73%) was observed against a lethal megalocytivirus challenge. Consistently, viral replication in the vaccinated fish was significantly inhibited. Immune response analysis showed that pORF75-vaccinated fish (i) exhibited upregulated expression of the genes involved in innate and adaptive immunity, (ii) possessed specific memory immune cells that showed significant response to secondary antigen stimulation, and (iii) produced specific serum antibodies which, when co-introduced into turbot with megalocytivirus, blocked viral replication. Furthermore, whole-genome transcriptome analysis revealed that ORF75 knockdown altered the transcription of 43 viral genes. Taken together, these results indicate that ORF75 encoded a highly protective immunogen that is also a global transcription regulator of megalocytivirus. (C) 2015 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000358626800033 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/49677]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jian,Li, Mo-fei. ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):486-494. |
| APA | Zhang, Jian,&Li, Mo-fei.(2015).ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate.FISH & SHELLFISH IMMUNOLOGY,45(2),486-494. |
| MLA | Zhang, Jian,et al."ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate".FISH & SHELLFISH IMMUNOLOGY 45.2(2015):486-494. |
入库方式： OAI收割
来源：海洋研究所
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