Tongue sole (Cynoglossus semilaevis) prothymosin alpha: Cytokine-like activities associated with the intact protein and the C-terminal region that lead to antiviral immunity via Myd88-dependent and -independent pathways respectively
文献类型：期刊论文
| 作者 | Zhang, Bao-cun; Sun, Li |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 53期号:1页码:96-104 |
| 关键词 | Cynoglossus semilaevis Prothymosin Antiviral Immunoregulation |
| 英文摘要 | Prothymosin alpha (ProT alpha) is a small protein that in mammals is known to participate in diverse biological processes including immunomodulation. In teleost, the immunological function of ProT alpha is unknown. In the current study, we investigated the expression and function of the ProT alpha (named CsProT alpha) from the teleost fish tongue sole (Cynoglossus semilaevis). We found that CsProT alpha expression was abundant in immune relevant tissues and upregulated by megalocytivirus infection. Immunoblot detected secretion of CsProT alpha by peripheral blood leukocytes. Recombinant CsProT alpha (rCsProT alpha) as well as the C-terminal 11-residue (Ct11) were able to bind head kidney monocytes (HKM) and induce immune gene expression; however, the induction patterns caused by rCsProT alpha and Ct11 differed considerably. When introduced in vivo, rCsProT alpha and Ctll significantly reduced megalocytivirus infection in fish tissues, whereas rCsProT alpha antibody significantly promoted viral replication. Blocking of Myd88 activity abolished the virus-inhibitory effect of rCsProT alpha but not Ct11. Taken together, these results demonstrate for the first time that both the intact protein and the C-terminal segment of a teleost ProT alpha can act like cytokines and induce antiviral immunity via, however, distinct signaling pathways that differ in the requirement of Myd88. (C) 2015 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000360772900012 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/50425]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Bao-cun,Sun, Li. Tongue sole (Cynoglossus semilaevis) prothymosin alpha: Cytokine-like activities associated with the intact protein and the C-terminal region that lead to antiviral immunity via Myd88-dependent and -independent pathways respectively[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,53(1):96-104. |
| APA | Zhang, Bao-cun,&Sun, Li.(2015).Tongue sole (Cynoglossus semilaevis) prothymosin alpha: Cytokine-like activities associated with the intact protein and the C-terminal region that lead to antiviral immunity via Myd88-dependent and -independent pathways respectively.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,53(1),96-104. |
| MLA | Zhang, Bao-cun,et al."Tongue sole (Cynoglossus semilaevis) prothymosin alpha: Cytokine-like activities associated with the intact protein and the C-terminal region that lead to antiviral immunity via Myd88-dependent and -independent pathways respectively".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 53.1(2015):96-104. |
入库方式： OAI收割
来源：海洋研究所
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