Isolation, expression and characterization of rbcL gene from Ulva prolifera J. Agardh (Ulvophyceae, Chlorophyta)
文献类型：期刊论文
| 作者 | Shao Zhanru1; Li Wei1,2; Guo Hui1,3; Duan Delin1 |
| 刊名 | JOURNAL OF OCEAN UNIVERSITY OF CHINA
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 14期号:6页码:1087-1095 |
| 关键词 | Ribulose-1 5-bisphosphate carboxylase/oxygenase large subunit sequence analysis real-time PCR in vitro expression Ulva prolifera |
| 英文摘要 | Ulva prolifera is a typical green alga in subtidal areas and can grow tremendously fast. A highly efficient Rubisco enzyme which is encoded by UpRbcL gene may contribute to the rapid growth. In this study, the full-length UpRbcL open reading frame (ORF) was identified, which encoded a protein of 474 amino acids. Phylogenetic analysis of UpRbcL sequences revealed that Chlorophyta had a closer genetic relationship with higher plants than with Rhodophyta and Phaeophyta. The two distinct residues (aa11 and aa91) were presumed to be unique for Rubisco catalytic activity. The predicted three-dimensional structure showed that one alpha/beta-barrel existed in the C-terminal region, and the sites for Mg2+ coordination and CO2 fixation were also located in this region. Gene expression profile indicated that UpRbcL was expressed at a higher level under light exposure than in darkness. When the culture temperature reached 35A degrees C, the expression level of UpRbcL was 2.5-fold lower than at 15A degrees C, and the carboxylase activity exhibited 13.8-fold decrease. UpRbcL was heterologously expressed in E. coli and was purified by Ni2+ affinity chromatography. The physiological and biochemical characterization of recombinant Rubisco will be explored in the future. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000364572000021 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/82291]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Jena, Inst Gen Bot & Plant Physiol, D-07743 Jena, Germany 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shao Zhanru,Li Wei,Guo Hui,et al. Isolation, expression and characterization of rbcL gene from Ulva prolifera J. Agardh (Ulvophyceae, Chlorophyta)[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2015,14(6):1087-1095. |
| APA | Shao Zhanru,Li Wei,Guo Hui,&Duan Delin.(2015).Isolation, expression and characterization of rbcL gene from Ulva prolifera J. Agardh (Ulvophyceae, Chlorophyta).JOURNAL OF OCEAN UNIVERSITY OF CHINA,14(6),1087-1095. |
| MLA | Shao Zhanru,et al."Isolation, expression and characterization of rbcL gene from Ulva prolifera J. Agardh (Ulvophyceae, Chlorophyta)".JOURNAL OF OCEAN UNIVERSITY OF CHINA 14.6(2015):1087-1095. |
入库方式： OAI收割
来源：海洋研究所
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