An LRR-only protein representing a new type of pattern recognition receptor in Chlamys farreri
文献类型：期刊论文
| 作者 | Wang, Mengqiang1 ; Wang, Lingling1,3; Guo, Ying1; Yi, Qilin2; Song, Linsheng2
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| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 54期号:1页码:145-155 |
| 关键词 | Chlamys farreri Innate immunity Leucine-rich repeat LRR-only protein Pattern recognition receptor |
| 英文摘要 | Accumulating evidence has demonstrated that leucine-rich repeat (LRR)-only proteins could mediate protein-ligand and protein protein interactions and were involved in the immune response. In the present study, an LRR-only protein (designed as CfLRRop-1) was cloned from Zhikong scallop Chlamys farreri. The complete cDNA sequence of CfLRRop-1 contained an open reading frame (ORF) of 1377 bp, which encoded a protein of 458 amino acids. An LRRNT motif, an LRR_7 motif and seven LRR motifs were found in the deduced amino acid sequence of CfLRRop-1. And these seven LRR motifs contained a conserved signature sequence LxxLxxNxL. The mRNA transcripts of CfLRRop-1 were constitutively expressed in all the tested tissues, including haemocytes, muscle, mantle, gill, hepatopancreas and gonad, with the highest expression level in hepatopancreas. After the stimulation of lipopolysaccharide (LPS), peptidoglycan (PGN), glucan (GLU) and polyinosinic-polycytidylic acid (poly I:C), the mRNA transcripts of CARRop-1 in haemocytes all increased firstly within the first 6 h and secondly during 12-24 h post stimulation. The mRNA expression level of CfLRRop-1 was continuously up-regulated, after the expression of CfTLR (previously identified Toll-like receptor in C. farreri) was suppressed via RNA interference (RNAi). The recombinant CfLRRop-1 protein could directly bind LPS, PGN, GLU and poly I:C, and induce the release of TNF-alpha in mixed primary cultured scallop haemocytes. These results collectively indicated that CfLRRop-1 would function as a powerful pattern recognition receptor (PRR) and play a pivotal role in the immune response of scallops. (C) 2015 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000364883100015 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/82299]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Mengqiang,Wang, Lingling,Guo, Ying,et al. An LRR-only protein representing a new type of pattern recognition receptor in Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,54(1):145-155. |
| APA | Wang, Mengqiang,Wang, Lingling,Guo, Ying,Yi, Qilin,&Song, Linsheng.(2016).An LRR-only protein representing a new type of pattern recognition receptor in Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,54(1),145-155. |
| MLA | Wang, Mengqiang,et al."An LRR-only protein representing a new type of pattern recognition receptor in Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 54.1(2016):145-155. |
入库方式： OAI收割
来源：海洋研究所
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