A suicidal zinc finger nuclease expression coupled with a surrogate reporter for efficient genome engineering
文献类型：期刊论文
| 作者 | Zhang, Cunfang1,2; Xu, Kun1; Hu, Linyong2; Wang, Ling1,3; Zhang, Tingting1; Ren, Chonghua1; Zhang, Zhiying1 |
| 刊名 | biotechnology letters
 |
| 出版日期 | 2015-02-01 |
| 卷号 | 37期号:2页码:299-305 |
| 关键词 | Cytotoxicity Double-strand-breaks Gene targeting Homologous recombination Off-targeting Zinc finger nuclease |
| 通讯作者 | zhang, zy (reprint author), northwest a&f univ, coll anim sci & technol, 22 xinong rd, yangling 712100, shaanxi, peoples r china. |
| 中文摘要 | genome editing with engineered nucleases, such as zinc-finger nucleases (zfns) and tale nucleases, remains confronted with a high risk of cellular toxicity induced by off-targeting. here we describe the construction of a suicidal nuclease expression vector in which a pair of zfns genes were flanked of its target sites. to further enrich the targeted cells, the suicidal zfn expression cassette was also inserted within an egfp reporter, to disrupt the orf of egfp gene. zfn-associated toxicity was reduced by similar to 40 % with this new system, and the activities of zfns were similar to 4.5 % lower. we conclude that using this new suicidal zfn expression and surrogate reporter system represents an improvement for genomic editing by reducing toxicity and allowing easy detection of edited cells by egfp analysis. |
| 英文摘要 | genome editing with engineered nucleases, such as zinc-finger nucleases (zfns) and tale nucleases, remains confronted with a high risk of cellular toxicity induced by off-targeting. here we describe the construction of a suicidal nuclease expression vector in which a pair of zfns genes were flanked of its target sites. to further enrich the targeted cells, the suicidal zfn expression cassette was also inserted within an egfp reporter, to disrupt the orf of egfp gene. zfn-associated toxicity was reduced by similar to 40 % with this new system, and the activities of zfns were similar to 4.5 % lower. we conclude that using this new suicidal zfn expression and surrogate reporter system represents an improvement for genomic editing by reducing toxicity and allowing easy detection of edited cells by egfp analysis. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | homologous recombination ; mammalian-cells ; gene ; drosophila |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000349230800006 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5457]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining, Peoples R China 3.Shaanxi Univ Technol, Sch Biol Sci Technol & Engn, Hanzhong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Cunfang,Xu, Kun,Hu, Linyong,et al. A suicidal zinc finger nuclease expression coupled with a surrogate reporter for efficient genome engineering[J]. biotechnology letters,2015,37(2):299-305. |
| APA | Zhang, Cunfang.,Xu, Kun.,Hu, Linyong.,Wang, Ling.,Zhang, Tingting.,...&Zhang, Zhiying.(2015).A suicidal zinc finger nuclease expression coupled with a surrogate reporter for efficient genome engineering.biotechnology letters,37(2),299-305. |
| MLA | Zhang, Cunfang,et al."A suicidal zinc finger nuclease expression coupled with a surrogate reporter for efficient genome engineering".biotechnology letters 37.2(2015):299-305. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。