Using Response Surface Methodology to Optimize Countercurrent Chromatographic Separation of Polyphenol Compounds from Fenugreek (Trigonella foenum-graecum L.) Seeds
文献类型：期刊论文
| 作者 | He, YF; Ding, CX; Wang, XY; Wang, HL; Suo, YR |
| 刊名 | journal of liquid chromatography & related technologies
 |
| 出版日期 | 2015 |
| 卷号 | 38期号:1页码:29-35 |
| 关键词 | fenugreek rhaponticin polyphenol compounds response surface methodology isovitexin high-speed countercurrent chromatography |
| 通讯作者 | wang, hl (reprint author), chinese acad sci, northwest inst plateau biol, key lab tibetan med res, xining 810001, peoples r china. |
| 中文摘要 | in this study, we used high-speed countercurrent chromatography (hsccc) to separate two bioactive polyphenols, rhaponticin and isovitexin, present in fenugreek (trigonella foenum-graecum l.) seeds. the hsccc process employed a two-phase solvent system composed of chloroform, methanol, water, and n-butanol in a 4:3:2:1.5 ratio (v:v:v:v). the hsccc separation conditions were optimized using response surface methodology (rsm). the optimal separation conditions were as follows: separation temperature, 26 degrees c; revolution speed, 874rpm; flow rate, 1.3ml/min. under these conditions, 21.6mg of rhaponticin and 12.8mg of isovitexin (>96.0% purity) were obtained from 100mg of the crude fenugreek seed extract. the purities and structures of the separated compounds were determined by high-performance liquid chromatography (hplc), and both proton and carbon nuclear magnetic resonance spectroscopy (h-1 nmr and c-13 nmr, respectively). the results demonstrate that (i) hsccc is an efficient technique for separating polyphenol compounds from seed extracts of fenugreek (trigonella foenum-graecum l.) and (ii) rsm is an effective approach for optimizing hsccc separation conditions. |
| 英文摘要 | in this study, we used high-speed countercurrent chromatography (hsccc) to separate two bioactive polyphenols, rhaponticin and isovitexin, present in fenugreek (trigonella foenum-graecum l.) seeds. the hsccc process employed a two-phase solvent system composed of chloroform, methanol, water, and n-butanol in a 4:3:2:1.5 ratio (v:v:v:v). the hsccc separation conditions were optimized using response surface methodology (rsm). the optimal separation conditions were as follows: separation temperature, 26 degrees c; revolution speed, 874rpm; flow rate, 1.3ml/min. under these conditions, 21.6mg of rhaponticin and 12.8mg of isovitexin (>96.0% purity) were obtained from 100mg of the crude fenugreek seed extract. the purities and structures of the separated compounds were determined by high-performance liquid chromatography (hplc), and both proton and carbon nuclear magnetic resonance spectroscopy (h-1 nmr and c-13 nmr, respectively). the results demonstrate that (i) hsccc is an efficient technique for separating polyphenol compounds from seed extracts of fenugreek (trigonella foenum-graecum l.) and (ii) rsm is an effective approach for optimizing hsccc separation conditions. |
| 收录类别 | SCI |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5464]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | He, YF,Ding, CX,Wang, XY,et al. Using Response Surface Methodology to Optimize Countercurrent Chromatographic Separation of Polyphenol Compounds from Fenugreek (Trigonella foenum-graecum L.) Seeds[J]. journal of liquid chromatography & related technologies,2015,38(1):29-35. |
| APA | He, YF,Ding, CX,Wang, XY,Wang, HL,&Suo, YR.(2015).Using Response Surface Methodology to Optimize Countercurrent Chromatographic Separation of Polyphenol Compounds from Fenugreek (Trigonella foenum-graecum L.) Seeds.journal of liquid chromatography & related technologies,38(1),29-35. |
| MLA | He, YF,et al."Using Response Surface Methodology to Optimize Countercurrent Chromatographic Separation of Polyphenol Compounds from Fenugreek (Trigonella foenum-graecum L.) Seeds".journal of liquid chromatography & related technologies 38.1(2015):29-35. |
入库方式： OAI收割
来源：西北高原生物研究所
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