Rapid Separation of Three Proanthocyanidin Dimers from Iris lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography With Continuous Sample Load and Double-Pump Balancing Mode
文献类型：期刊论文
| 作者 | Lv, Huanhuan1,2; Yuan, Zhenzhen1,2; Wang, Xiaoyan1; Wang, Zhenhua1; Suo, Yourui1; Wang, Honglun1 |
| 刊名 | phytochemical analysis
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 26期号:6页码:444-453 |
| 关键词 | Iris lactea Pall var chinensis (Fisch.) Koidz proanthocyanidins HSCCC continuous sample load double-pump balancing mode |
| 中文摘要 | introduction - the dried seeds of iris lactea have been used in traditional chinese medicine. previous studies have been focused on irisquinones while other chemical components are rarely reported. objective - to establish an efficient high-speed counter-current chromatography (hsccc) separation method with continuous sample load (csl) and double-pump balancing (dpb) mode to isolate proanthocyanidins from i. lactea. methods - firstly, an ethyl acetate extract of i. lacteawas pre-fractionated by silica column chromatography for the enrichment of proanthocyanidins. secondly, the enriched proanthocyanidins sample ( eps) was further fractionated by hsccc with a two-phase solvent system ethyl acetate: n- butanol: water ( 9: 1: 10, v/ v/ v) using dpb mode. the flow rate of the two phases was 2.2ml/ min, the revolution speed was 900 rpm, the separation temperature was 30 degrees c and the detection wavelength was 280nm. finally, the structures of the three isolated proanthocyanidins were elucidated by spectroscopic methods and compared with published data. results - under the optimized conditions, 600mg of the eps with six continuous injections ( 100mg/ time) was fractionated, yielding 57mg of prodelphinidin b3, 198mg of procyanidin b3, and 162mg of procyanidin b1, at purities of 97.2%, 98.1% and 97.3%, respectively. conclusions - the hsccc separation method with csl and dpb proved to be rapid, convenient and economical, constituting an efficient strategy for the isolation of proanthocyanidins. copyright (c) 2015 john wiley & sons, ltd. |
| 英文摘要 | introduction - the dried seeds of iris lactea have been used in traditional chinese medicine. previous studies have been focused on irisquinones while other chemical components are rarely reported. objective - to establish an efficient high-speed counter-current chromatography (hsccc) separation method with continuous sample load (csl) and double-pump balancing (dpb) mode to isolate proanthocyanidins from i. lactea. methods - firstly, an ethyl acetate extract of i. lacteawas pre-fractionated by silica column chromatography for the enrichment of proanthocyanidins. secondly, the enriched proanthocyanidins sample ( eps) was further fractionated by hsccc with a two-phase solvent system ethyl acetate: n- butanol: water ( 9: 1: 10, v/ v/ v) using dpb mode. the flow rate of the two phases was 2.2ml/ min, the revolution speed was 900 rpm, the separation temperature was 30 degrees c and the detection wavelength was 280nm. finally, the structures of the three isolated proanthocyanidins were elucidated by spectroscopic methods and compared with published data. results - under the optimized conditions, 600mg of the eps with six continuous injections ( 100mg/ time) was fractionated, yielding 57mg of prodelphinidin b3, 198mg of procyanidin b3, and 162mg of procyanidin b1, at purities of 97.2%, 98.1% and 97.3%, respectively. conclusions - the hsccc separation method with csl and dpb proved to be rapid, convenient and economical, constituting an efficient strategy for the isolation of proanthocyanidins. copyright (c) 2015 john wiley & sons, ltd. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; plant sciences ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; plant sciences ; chemistry |
| 关键词[WOS] | preparative isolation ; purification ; procyanidins ; hplc ; biotransformation ; identification ; flavan-3-ols ; extraction ; components ; leaves |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000363332400008 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5491]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, Huanhuan,Yuan, Zhenzhen,Wang, Xiaoyan,et al. Rapid Separation of Three Proanthocyanidin Dimers from Iris lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography With Continuous Sample Load and Double-Pump Balancing Mode[J]. phytochemical analysis,2015,26(6):444-453. |
| APA | Lv, Huanhuan,Yuan, Zhenzhen,Wang, Xiaoyan,Wang, Zhenhua,Suo, Yourui,&Wang, Honglun.(2015).Rapid Separation of Three Proanthocyanidin Dimers from Iris lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography With Continuous Sample Load and Double-Pump Balancing Mode.phytochemical analysis,26(6),444-453. |
| MLA | Lv, Huanhuan,et al."Rapid Separation of Three Proanthocyanidin Dimers from Iris lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography With Continuous Sample Load and Double-Pump Balancing Mode".phytochemical analysis 26.6(2015):444-453. |
入库方式： OAI收割
来源：西北高原生物研究所
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