Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction-Derivatization
文献类型：期刊论文
| 作者 | Liu, Mengge1,2; Chen, Guang1,2,3; Guo, Hailong1,2; Fan, Baoki4; Liu, Jianjun1,2; Fu, Qiang5; Li, Xiu1,2; Lu, Xiaomin1,2; Zhao, Xianen1,2; Li, Guoliang1,2 |
| 刊名 | journal of agricultural and food chemistry
 |
| 出版日期 | 2015-09-16 |
| 卷号 | 63期号:36页码:8058-8067 |
| 关键词 | plant growth regulators (PGRs) microwave-assisted extraction-derivatization (MAED) transgenic food signal transduction system (STS) |
| 中文摘要 | determination of plant growth regulators (pgrs) in a signal transduction system (sts) is significant for transgenic food safety, but may be challenged by poor accuracy and analyte instability. in this work, a microwave-assisted extraction-derivatization (maed) method is developed for six acidic pgrs in oil samples, allowing an efficient (<1.5 h) and facile (one step) pretreatment. accuracies are greatly improved, particularly for gibberellin a(3) (-2.72 to -0.65%) as compared with those reported (-22 to -2%). excellent selectivity and quite low detection limits (0.37-1.36 ng ml(-1)) are enabled by fluorescence detection-mass spectrum monitoring. results show the significant differences in acidic pgrs between transgenic and nontransgenic oils, particularly 1-naphthaleneacetic acid (1-naa), implying the pgrs induced variations of components and genes. this study provides, for the first time, an accurate and efficient determination for labile pgrs involved in sts and a promising concept for objectively evaluating the safety of transgenic foods. |
| 英文摘要 | determination of plant growth regulators (pgrs) in a signal transduction system (sts) is significant for transgenic food safety, but may be challenged by poor accuracy and analyte instability. in this work, a microwave-assisted extraction-derivatization (maed) method is developed for six acidic pgrs in oil samples, allowing an efficient (<1.5 h) and facile (one step) pretreatment. accuracies are greatly improved, particularly for gibberellin a(3) (-2.72 to -0.65%) as compared with those reported (-22 to -2%). excellent selectivity and quite low detection limits (0.37-1.36 ng ml(-1)) are enabled by fluorescence detection-mass spectrum monitoring. results show the significant differences in acidic pgrs between transgenic and nontransgenic oils, particularly 1-naphthaleneacetic acid (1-naa), implying the pgrs induced variations of components and genes. this study provides, for the first time, an accurate and efficient determination for labile pgrs involved in sts and a promising concept for objectively evaluating the safety of transgenic foods. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | agriculture, multidisciplinary ; chemistry, applied ; food science & technology |
| 研究领域[WOS] | agriculture ; chemistry ; food science & technology |
| 关键词[WOS] | performance liquid-chromatography ; tandem mass-spectrometry ; induced fluorescence detection ; chemical derivatization ; gene-expression ; alpha-amylase ; abscisic-acid ; capillary-electrophoresis ; major phytohormones ; jasmonic acid |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000361502000021 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5507]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China 4.Hubei Univ Sci & Technol, Xianning 437100, Peoples R China 5.Qinghai Normal Univ, Xining 810008, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Mengge,Chen, Guang,Guo, Hailong,et al. Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction-Derivatization[J]. journal of agricultural and food chemistry,2015,63(36):8058-8067. |
| APA | Liu, Mengge.,Chen, Guang.,Guo, Hailong.,Fan, Baoki.,Liu, Jianjun.,...&You, Jinmao.(2015).Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction-Derivatization.journal of agricultural and food chemistry,63(36),8058-8067. |
| MLA | Liu, Mengge,et al."Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction-Derivatization".journal of agricultural and food chemistry 63.36(2015):8058-8067. |
入库方式： OAI收割
来源：西北高原生物研究所
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