Pronuciferine and nuciferine inhibit lipogenesis in 3T3-L1 adipocytes by activating the AMPK signaling pathway
文献类型：期刊论文
| 作者 | Ma, Chengjun1; Li, Gang1,2; He, Yanfeng2; Xu, Bo1,3; Mi, Xiangquan4; Wang, Honglun2; Wang, Zhenhua1 |
| 刊名 | life sciences
 |
| 出版日期 | 2015-09-01 |
| 卷号 | 136页码:120-125 |
| 关键词 | 3T3-L1 adipocytes Pronuciferine Nuciferine Lipogenesis AMPK |
| 中文摘要 | aims: nelumbo nucifera (gaertn.) leaves are used widely in modulating obesity in traditional chinese medicine. our previous work demonstrated that aporphine alkaloids from it increased the glucose consumption in mature 3t3-l1 adipocytes. however, the underlying mechanisms of this increase remain unclear. here we investigated the modulating effects of pronuciferine and nuciferine on lipogenesis and glucose uptake in insulin resistant 3t3-l1 adipocytes in vitro. main methods: insulin resistant 3t3-l1 mature adipocytes were induced with dexamethasone, 3-isobutyl-methylxanthine and insulin. the lipid droplets and the intracellular triglyceride contents in mature adipocytes were detected by oil red o staining and colorimetry respectively. the glucose uptake was measured with a fluorescent deoxyglucose analog (2-nbdg). the glucose transporter type 4 (glut-4) expression was measured by fluorescent-immunohistochemistry and the activation of 5'-amp-activated protein kinase (ampk) was detected by its alpha subunit phosphorylation. key findings: both nuciferine and pronuciferine treatments significantly decreased the lipid droplets and the intracellular triglyceride contents but increased the glucose uptake in the insulin resistant 3t3-l1 adipocytes. furthermore, both pronuciferine and nuciferine showed the ability to up-regulate the expression of glut4, triggering the phosphorylation of ampk in mature 3t3-l1 adipocytes, although pronuciferine exhibited a more powerful effect compared to nuciferine. significance: in summary, all the results demonstrate that pronuciferine and nuciferine ameliorate the glucose and lipid metabolism in insulin-resistant 3t3-l1 adipocytes, which might be due to the activation of the ampk signaling pathway. (c) 2015 elsevier inc. all rights reserved. |
| 英文摘要 | aims: nelumbo nucifera (gaertn.) leaves are used widely in modulating obesity in traditional chinese medicine. our previous work demonstrated that aporphine alkaloids from it increased the glucose consumption in mature 3t3-l1 adipocytes. however, the underlying mechanisms of this increase remain unclear. here we investigated the modulating effects of pronuciferine and nuciferine on lipogenesis and glucose uptake in insulin resistant 3t3-l1 adipocytes in vitro. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | medicine, research & experimental ; pharmacology & pharmacy |
| 研究领域[WOS] | research & experimental medicine ; pharmacology & pharmacy |
| 关键词[WOS] | induced insulin-resistance ; nelumbo-nucifera ; protein-kinase ; lipid-metabolism ; glucose ; leaves ; mice ; differentiation ; resveratrol ; expression |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000361035500017 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5516]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Yantai Univ, Sch Life Sci, Ctr Mitochondria & Healthy Aging, Yantai 264005, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 3.Yantai Univ, Sch Pharm, Key Lab Mol Pharmacol & Drug Evaluat, Minist Educ China, Yantai 264005, Peoples R China 4.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Chengjun,Li, Gang,He, Yanfeng,et al. Pronuciferine and nuciferine inhibit lipogenesis in 3T3-L1 adipocytes by activating the AMPK signaling pathway[J]. life sciences,2015,136:120-125. |
| APA | Ma, Chengjun.,Li, Gang.,He, Yanfeng.,Xu, Bo.,Mi, Xiangquan.,...&Wang, Zhenhua.(2015).Pronuciferine and nuciferine inhibit lipogenesis in 3T3-L1 adipocytes by activating the AMPK signaling pathway.life sciences,136,120-125. |
| MLA | Ma, Chengjun,et al."Pronuciferine and nuciferine inhibit lipogenesis in 3T3-L1 adipocytes by activating the AMPK signaling pathway".life sciences 136(2015):120-125. |
入库方式： OAI收割
来源：西北高原生物研究所
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