Isolation and characterization of 15 polymorphic genomic simple sequence repeat markers in Poa pratensis L.
文献类型：期刊论文
| 作者 | Zhao, Yanyan1,2; Yu, Feng1,2; Li, Yuan1,2; Wang, Haiqing1; Dou, Quanwen1 |
| 刊名 | grassland science
 |
| 出版日期 | 2015-09-01 |
| 卷号 | 61期号:3页码:185-187 |
| 关键词 | Genomic simple sequence repeat Poa pratensis polymorphism |
| 中文摘要 | poa pratensis l. is an important perennial grass used both for forage and turf in temperate regions of the world. in this study, we isolated and characterized 15 novel polymorphic genomic simple sequence repeat (ssr) markers in p.pratensis. the polymorphism of each marker was assessed in 14 cultivars. the number of alleles of individual microsatellites ranged from three to six, with an average of 3.8 alleles per ssr. cross-species amplification tests showed that 13 and seven of the characterized p.pratensis markers could be effectively amplified in p.pratensis var. anceps gaud and p.crymophila keng, respectively. these polymorphic microsatellite markers should serve as powerful tools for the investigation of genetic diversity and related analyses, and also for molecular-assisted breeding of p.pratensis. |
| 英文摘要 | poa pratensis l. is an important perennial grass used both for forage and turf in temperate regions of the world. in this study, we isolated and characterized 15 novel polymorphic genomic simple sequence repeat (ssr) markers in p.pratensis. the polymorphism of each marker was assessed in 14 cultivars. the number of alleles of individual microsatellites ranged from three to six, with an average of 3.8 alleles per ssr. cross-species amplification tests showed that 13 and seven of the characterized p.pratensis markers could be effectively amplified in p.pratensis var. anceps gaud and p.crymophila keng, respectively. these polymorphic microsatellite markers should serve as powerful tools for the investigation of genetic diversity and related analyses, and also for molecular-assisted breeding of p.pratensis. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | agriculture, multidisciplinary ; agronomy |
| 研究领域[WOS] | agriculture |
| 关键词[WOS] | microsatellite markers |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000360717700008 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5517]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Yanyan,Yu, Feng,Li, Yuan,et al. Isolation and characterization of 15 polymorphic genomic simple sequence repeat markers in Poa pratensis L.[J]. grassland science,2015,61(3):185-187. |
| APA | Zhao, Yanyan,Yu, Feng,Li, Yuan,Wang, Haiqing,&Dou, Quanwen.(2015).Isolation and characterization of 15 polymorphic genomic simple sequence repeat markers in Poa pratensis L..grassland science,61(3),185-187. |
| MLA | Zhao, Yanyan,et al."Isolation and characterization of 15 polymorphic genomic simple sequence repeat markers in Poa pratensis L.".grassland science 61.3(2015):185-187. |
入库方式： OAI收割
来源：西北高原生物研究所
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