Characterization of two paralogous myostatin genes and evidence for positive selection in Tibet fish: Gymnocypris przewalskii
文献类型：期刊论文
| 作者 | Tong, Chao1,2; Zhang, Cunfang1; Shi, Jianquan3; Qi, Hongfang3; Zhang, Renyi1,2; Tang, Yongtao1,2; Li, Guogang1,2; Feng, Chenguang1,2; Zhao, Kai1 |
| 刊名 | gene
 |
| 出版日期 | 2015-07-10 |
| 卷号 | 565期号:2页码:201-210 |
| 关键词 | Gymnocypris przewalskii Myostatin Paralogue Gene duplication Positive selection Expression pattern |
| 中文摘要 | myostatin (mstn) is an important member of tgf-beta superfamily, a muscle growth inhibitor. though mstn has been identified in many organisms, little is known about this gene in highland fish, gymnocypris przewalskii endemic to the qinghai-tibetan plateau. in this study, we first cloned two paralogous mstn genes (mstn1 and mstn2) from g. przewalskii through homologue cloning. the 3d structures of both mstn proteins varied in the numbers of-sheets and conformations of alpha-helices. the branch-site model showed that mstn1 has undergone positive selection, and two positively selected sites (107m and 181t) were located on the random coils of the 3d protein structure. expression patterns indicated that the mstn1 expressed widely, while the mstn2 only expressed in the muscle and brain. during the early stage of embryo development, the expression levels of both mstn paralogous genes showed different increasing trends. these results suggest that it is diverging in two mstn paralogues of g. przewalskii via specific differences in gene structure, protein structure, selection pressure and gene expression patterns. taken together, this study provides novel contribution on the research topics of growth related gene function and mechanism of highland fish in extreme aquatic environment on the qinghai-tibetan plateau. (c) 2015 elsevier b.v. all rights reserved. |
| 英文摘要 | myostatin (mstn) is an important member of tgf-beta superfamily, a muscle growth inhibitor. though mstn has been identified in many organisms, little is known about this gene in highland fish, gymnocypris przewalskii endemic to the qinghai-tibetan plateau. in this study, we first cloned two paralogous mstn genes (mstn1 and mstn2) from g. przewalskii through homologue cloning. the 3d structures of both mstn proteins varied in the numbers of-sheets and conformations of alpha-helices. the branch-site model showed that mstn1 has undergone positive selection, and two positively selected sites (107m and 181t) were located on the random coils of the 3d protein structure. expression patterns indicated that the mstn1 expressed widely, while the mstn2 only expressed in the muscle and brain. during the early stage of embryo development, the expression levels of both mstn paralogous genes showed different increasing trends. these results suggest that it is diverging in two mstn paralogues of g. przewalskii via specific differences in gene structure, protein structure, selection pressure and gene expression patterns. taken together, this study provides novel contribution on the research topics of growth related gene function and mechanism of highland fish in extreme aquatic environment on the qinghai-tibetan plateau. (c) 2015 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | genetics & heredity |
| 研究领域[WOS] | genetics & heredity |
| 关键词[WOS] | differential expression ; phylogenetic analysis ; maximum-likelihood ; genome duplication ; zebrafish ; growth ; evolution ; muscle ; trout ; mutations |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000355711800007 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5535]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Rescues Ctr Qinghai Lake Naked Carp, Xining 810016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tong, Chao,Zhang, Cunfang,Shi, Jianquan,et al. Characterization of two paralogous myostatin genes and evidence for positive selection in Tibet fish: Gymnocypris przewalskii[J]. gene,2015,565(2):201-210. |
| APA | Tong, Chao.,Zhang, Cunfang.,Shi, Jianquan.,Qi, Hongfang.,Zhang, Renyi.,...&Zhao, Kai.(2015).Characterization of two paralogous myostatin genes and evidence for positive selection in Tibet fish: Gymnocypris przewalskii.gene,565(2),201-210. |
| MLA | Tong, Chao,et al."Characterization of two paralogous myostatin genes and evidence for positive selection in Tibet fish: Gymnocypris przewalskii".gene 565.2(2015):201-210. |
入库方式： OAI收割
来源：西北高原生物研究所
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