The influence of organic sample solvents on the separation efficiency of basic compounds under strong cation exchange mode
文献类型：期刊论文
| 作者 | Long, Zhen1; Yu, Dongping1; Liu, Yanfang1; Du, Nana1; Tao, Yanduo2; Mei, Lijuan2; Guo, Zhimou1; Liang, Xinmiao1 |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2015-05-04 |
| 卷号 | 872页码:77-83 |
| 关键词 | Strong cation exchange Separation efficiency Solvent elution strength Solvent injection volume |
| 中文摘要 | this study investigated the influence of organic sample solvents on separation efficiency of basic compounds under strong cation exchange (scx) mode. the mixtures of acidic aqueous solution and organic solvent such as acetonitrile, ethanol, methanol and dimethyl sulfoxide (dmso) were tested as sample solvents. for later-eluting analytes, the increase of sample solvent elution strength was responsible for the decrease of separation efficiency. thus, sample solvents with weak elution strength could provide high separation efficiencies. for earlier-eluting analytes, the retention of organic sample solvents was the main factor affecting separation efficiency. weakly retained solvents could provide high separation efficiency. in addition, an optimized approach was proposed to reduce the effect of organic sample solvent, in which low ionic solvent was employed as initial mobile phase in the gradient. at last, the analysis of impurities in hydrophobic drug berberine was performed. the results showed that using acidic aqueous methanol as sample solvents could provide high separation efficiency and good resolution (r > 1.5). (c) 2015 elsevier b.v. all rights reserved. |
| 英文摘要 | this study investigated the influence of organic sample solvents on separation efficiency of basic compounds under strong cation exchange (scx) mode. the mixtures of acidic aqueous solution and organic solvent such as acetonitrile, ethanol, methanol and dimethyl sulfoxide (dmso) were tested as sample solvents. for later-eluting analytes, the increase of sample solvent elution strength was responsible for the decrease of separation efficiency. thus, sample solvents with weak elution strength could provide high separation efficiencies. for earlier-eluting analytes, the retention of organic sample solvents was the main factor affecting separation efficiency. weakly retained solvents could provide high separation efficiency. in addition, an optimized approach was proposed to reduce the effect of organic sample solvent, in which low ionic solvent was employed as initial mobile phase in the gradient. at last, the analysis of impurities in hydrophobic drug berberine was performed. the results showed that using acidic aqueous methanol as sample solvents could provide high separation efficiency and good resolution (r > 1.5). (c) 2015 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; reversed stationary phases ; injection volume ; mobile-phase ; peak shape ; drugs ; berberine ; hplc ; identification ; purification |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000353218600009 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5558]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Peoples R China |
| 推荐引用方式 GB/T 7714 | Long, Zhen,Yu, Dongping,Liu, Yanfang,et al. The influence of organic sample solvents on the separation efficiency of basic compounds under strong cation exchange mode[J]. analytica chimica acta,2015,872:77-83. |
| APA | Long, Zhen.,Yu, Dongping.,Liu, Yanfang.,Du, Nana.,Tao, Yanduo.,...&Liang, Xinmiao.(2015).The influence of organic sample solvents on the separation efficiency of basic compounds under strong cation exchange mode.analytica chimica acta,872,77-83. |
| MLA | Long, Zhen,et al."The influence of organic sample solvents on the separation efficiency of basic compounds under strong cation exchange mode".analytica chimica acta 872(2015):77-83. |
入库方式： OAI收割
来源：西北高原生物研究所
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