Separation and purification of four oligostilbenes from Iris lactea Pall. var. chinensis (Fisch.) Koidz by high-speed counter-current chromatography
文献类型：期刊论文
| 作者 | Lv, Huanhuan1,2; Wang, Honglun1; He, Yanfeng1,2; Ding, Chenxu1; Wang, Xiaoyan1; Suo, Yourui1 |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2015-04-15 |
| 卷号 | 988页码:127-134 |
| 关键词 | HSCCC Iris lactea Oligostilbenes Preparation |
| 中文摘要 | a method of using high-speed counter-current chromatography (hsccc) for preparative isolation and purification of oligostilbenes from the ethanol extracts of seed kernel of iris lactea pall. var. chinensis (fisch.) koidz was established in this study. four oligostilbenes were successfully separated and purified by hsccc with two sets of two-phase solvent system, n-hexane-ethyl acetate-methanol-water (3:6:4.2:5.5, v/v/v/v) in the head-to-tail elution mode for the first separation to mainly isolate vitisin a (58 mg), epsilon-viniferin (76 mg) and peak ii (43 mg) from 300 mg of the crude ethanol extracts, and then light petroleum ethyl acetate methanol water (5:5:3:6, v/v/v/v) in the tail-to-head elution mode for the second separation to isolate vitisin b (52 mg) and vitisin c (11 mg) from 100 mg of peak ii. the purities of the isolated four oligostilbenes were all over 95.0% as determined by hplc. vitisin a, vitisin b and vitisin c, resveratrol tetramers, were isolated from iris lactea for the first time. the preparation of crude sample was simple and the hsccc method for the isolation and purification of four oligostilbenes was rapid, efficient and economical. (c) 2015 elsevier b.v. all rights reserved. |
| 英文摘要 | a method of using high-speed counter-current chromatography (hsccc) for preparative isolation and purification of oligostilbenes from the ethanol extracts of seed kernel of iris lactea pall. var. chinensis (fisch.) koidz was established in this study. four oligostilbenes were successfully separated and purified by hsccc with two sets of two-phase solvent system, n-hexane-ethyl acetate-methanol-water (3:6:4.2:5.5, v/v/v/v) in the head-to-tail elution mode for the first separation to mainly isolate vitisin a (58 mg), epsilon-viniferin (76 mg) and peak ii (43 mg) from 300 mg of the crude ethanol extracts, and then light petroleum ethyl acetate methanol water (5:5:3:6, v/v/v/v) in the tail-to-head elution mode for the second separation to isolate vitisin b (52 mg) and vitisin c (11 mg) from 100 mg of peak ii. the purities of the isolated four oligostilbenes were all over 95.0% as determined by hplc. vitisin a, vitisin b and vitisin c, resveratrol tetramers, were isolated from iris lactea for the first time. the preparation of crude sample was simple and the hsccc method for the isolation and purification of four oligostilbenes was rapid, efficient and economical. (c) 2015 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | vitis-vinifera kyohou ; heyneanol-a ; amurensis ; roots ; seeds ; leaf ; stem ; resveratrol ; cells |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352328600018 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5562]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, Huanhuan,Wang, Honglun,He, Yanfeng,et al. Separation and purification of four oligostilbenes from Iris lactea Pall. var. chinensis (Fisch.) Koidz by high-speed counter-current chromatography[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2015,988:127-134. |
| APA | Lv, Huanhuan,Wang, Honglun,He, Yanfeng,Ding, Chenxu,Wang, Xiaoyan,&Suo, Yourui.(2015).Separation and purification of four oligostilbenes from Iris lactea Pall. var. chinensis (Fisch.) Koidz by high-speed counter-current chromatography.journal of chromatography b-analytical technologies in the biomedical and life sciences,988,127-134. |
| MLA | Lv, Huanhuan,et al."Separation and purification of four oligostilbenes from Iris lactea Pall. var. chinensis (Fisch.) Koidz by high-speed counter-current chromatography".journal of chromatography b-analytical technologies in the biomedical and life sciences 988(2015):127-134. |
入库方式： OAI收割
来源：西北高原生物研究所
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