Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection
文献类型：期刊论文
| 作者 | Wu, Hongliang1,4; Li, Guoliang2; Liu, Shucheng3; Liu, Di1,4; Chen, Guang2; Hu, Na1,4; Suo, Yourui1; You, Jinmao1,2 |
| 刊名 | journal of pharmaceutical and biomedical analysis
 |
| 出版日期 | 2015-03-25 |
| 卷号 | 107页码:98-107 |
| 关键词 | Triterpenic acids Pre-column derivatization High-performance liquid chromatography Ultrasound-assisted dispersive liquid-liquid microextraction Response surface methodology Traditional Chinese medicinal herbs |
| 中文摘要 | a novel analytical method was developed for simultaneous determination of six triterpenic acids using ultrasound-assisted dispersive liquid-liquid microextraction (ua-dllme) follow by high-performance liquid chromatography (hplc) with fluorescence detection. six triterpenic acids (ursolic acid, oleanolic acid, betulinic acid, maslinic acid, betulonic acid and corosolic acid) were extracted by ua-dllme using chloroform and acetone as the extraction and disperser solvents, respectively. after the extraction and nitrogen flushing, the extracts were rapidly derivatized with 2-(12,13-dihydro-7h-dibenzo[a,g]carbazol-7-yl)ethyl4-methylbenzenesulfonate. the main experimental parameters affecting extraction efficiency and derivatization yield were investigated and optimized by response surface methodology (rsm) combined with box-behnken design (bbd). the limits of detection (lods) and the limits of quantification (loqs) were in the range of 0.95-1.36 ng ml(-1) and 3.17-4.55 ng ml(-1), respectively. under the optimum conditions, the method has been successfully applied for the analysis of triterpenic acids in six different traditional chinese medicinal herbs. (c) 2014 elsevier b.v. all rights reserved. |
| 英文摘要 | a novel analytical method was developed for simultaneous determination of six triterpenic acids using ultrasound-assisted dispersive liquid-liquid microextraction (ua-dllme) follow by high-performance liquid chromatography (hplc) with fluorescence detection. six triterpenic acids (ursolic acid, oleanolic acid, betulinic acid, maslinic acid, betulonic acid and corosolic acid) were extracted by ua-dllme using chloroform and acetone as the extraction and disperser solvents, respectively. after the extraction and nitrogen flushing, the extracts were rapidly derivatized with 2-(12,13-dihydro-7h-dibenzo[a,g]carbazol-7-yl)ethyl4-methylbenzenesulfonate. the main experimental parameters affecting extraction efficiency and derivatization yield were investigated and optimized by response surface methodology (rsm) combined with box-behnken design (bbd). the limits of detection (lods) and the limits of quantification (loqs) were in the range of 0.95-1.36 ng ml(-1) and 3.17-4.55 ng ml(-1), respectively. under the optimum conditions, the method has been successfully applied for the analysis of triterpenic acids in six different traditional chinese medicinal herbs. (c) 2014 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences ; life sciences & biomedicine |
| 类目[WOS] | chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | capillary-zone-electrophoresis ; thin-layer-chromatography ; solid-phase extraction ; ursolic acid ; oleanolic acid ; mass-spectrometry ; labeling reagent ; fatty-acids ; hplc ; derivatization |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351116900014 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5572]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China 3.Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China 4.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Hongliang,Li, Guoliang,Liu, Shucheng,et al. Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection[J]. journal of pharmaceutical and biomedical analysis,2015,107:98-107. |
| APA | Wu, Hongliang.,Li, Guoliang.,Liu, Shucheng.,Liu, Di.,Chen, Guang.,...&You, Jinmao.(2015).Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection.journal of pharmaceutical and biomedical analysis,107,98-107. |
| MLA | Wu, Hongliang,et al."Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection".journal of pharmaceutical and biomedical analysis 107(2015):98-107. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。