Determination of Plant Growth Regulators in Vegetable by High Performance Liquid Chromatography-Tandem Mass Spectrometry Coupled with Isotop-coded Derivatization
文献类型：期刊论文
| 作者 | Cai Yi-Ping1; Sun Zhi-Wei1; Wang Xiao-Yan2; Suo You-Rui2; You Jin-Mao1,2 |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2015-03-01 |
| 卷号 | 43期号:3页码:419-423 |
| 关键词 | Isotope-coded derivatization High performance liquid chromatography-tandem mass spectrometry Plant growth regulators |
| 中文摘要 | a method for determination of 6 plant growth regulators by high performance liquid chromatography-tandem mass spectrometry (hplc-ms/ms) coupled with isotope-coded derivatization was developed. d(0)-10-methyl-acridone-2-sulfonyl piperazine (d(0)-maspz, light form) and d(3)-10-methyl-acridone-2-sulfonyl piperazine (d(3)-maspz, heavy form) were prepared as isotope-coded derivatization reagents for carboxyl compound. the carboxylic plant growth regulator standards and real samples were derivatized by d(0)-maspz and d(3)-maspz, respectively. the obtained solutions were mixed at a certain ratio, and then injected for hplc-ms/ms analysis. the light and heavy derivatives were monitored with transitions of [m + h](+)-> m/z 208.2 and [m + h](+)-> m/z 211.2, respectively. with heavy derivative as internal standard for corresponding light derivative, the global isotope internal standard quantification for 6 plant growth regulators was achieved. the results indicated that the proposed isotope-coded derivatization method could provide relative quantitative data with adequate linearity in a 10-fold dynamic range (r = 0.9991). the detection and quantitation limits were 0.19-0.34 mu m and 0.53-0.96 mu m, respectively. the relative standard deviations were <= 3.8%, and the accuracies ranged from 97. 5% to 103.8%. |
| 英文摘要 | a method for determination of 6 plant growth regulators by high performance liquid chromatography-tandem mass spectrometry (hplc-ms/ms) coupled with isotope-coded derivatization was developed. d(0)-10-methyl-acridone-2-sulfonyl piperazine (d(0)-maspz, light form) and d(3)-10-methyl-acridone-2-sulfonyl piperazine (d(3)-maspz, heavy form) were prepared as isotope-coded derivatization reagents for carboxyl compound. the carboxylic plant growth regulator standards and real samples were derivatized by d(0)-maspz and d(3)-maspz, respectively. the obtained solutions were mixed at a certain ratio, and then injected for hplc-ms/ms analysis. the light and heavy derivatives were monitored with transitions of [m + h](+)-> m/z 208.2 and [m + h](+)-> m/z 211.2, respectively. with heavy derivative as internal standard for corresponding light derivative, the global isotope internal standard quantification for 6 plant growth regulators was achieved. the results indicated that the proposed isotope-coded derivatization method could provide relative quantitative data with adequate linearity in a 10-fold dynamic range (r = 0.9991). the detection and quantitation limits were 0.19-0.34 mu m and 0.53-0.96 mu m, respectively. the relative standard deviations were <= 3.8%, and the accuracies ranged from 97. 5% to 103.8%. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | reagent ; samples |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351233800004 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5575]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai Yi-Ping,Sun Zhi-Wei,Wang Xiao-Yan,et al. Determination of Plant Growth Regulators in Vegetable by High Performance Liquid Chromatography-Tandem Mass Spectrometry Coupled with Isotop-coded Derivatization[J]. chinese journal of analytical chemistry,2015,43(3):419-423. |
| APA | Cai Yi-Ping,Sun Zhi-Wei,Wang Xiao-Yan,Suo You-Rui,&You Jin-Mao.(2015).Determination of Plant Growth Regulators in Vegetable by High Performance Liquid Chromatography-Tandem Mass Spectrometry Coupled with Isotop-coded Derivatization.chinese journal of analytical chemistry,43(3),419-423. |
| MLA | Cai Yi-Ping,et al."Determination of Plant Growth Regulators in Vegetable by High Performance Liquid Chromatography-Tandem Mass Spectrometry Coupled with Isotop-coded Derivatization".chinese journal of analytical chemistry 43.3(2015):419-423. |
入库方式： OAI收割
来源：西北高原生物研究所
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