A new primer-pair for sex identification of larks and wagtails
文献类型：期刊论文
| 作者 | Chu, Hui1,2; Guo, Xinyi1,2; Zeng, Yan3; Zou, Xiaoyan1; Guo, Songchang1,4; Zhao, Liang1 |
| 刊名 | conservation genetics resources
 |
| 出版日期 | 2015-03-01 |
| 卷号 | 7期号:1页码:19-21 |
| 关键词 | Sex identification CHD1 Larks Wagtails |
| 中文摘要 | chromobox-helicase dna-binding gene (chd1) is the most reliable gene for sex identification in birds. however, the chd1 fragments of some species are difficult to amplify. in this study, we designed a new primer set (intp2/intp8) that targets a conserved region of chd1 gene. firstly, we tested this protocol in oriental skylark (alauda gulgula). pcr amplification produced a single band (259 bp) for males and two bands (259 and 297 bp) for females. we then successfully conducted sex identification in other bird species including tibetan lark (melanocorypha maxima), horned lark (eremophila alpestris), mongolian skylark (melanocorypha mongolica), asian short-toed lark (calandrella cheleensis), humes short-toed lark (calandrella acutirostris), crested lark (galerida cristata), yellow-headed wagtail (motacilla citreola), white wagtail (motacilla alba) and ground tit (pseudopodoces humilis hume). collectively, these findings support that intp2/intp8 primer set can be used to accurately determine sex identity in birds. |
| 英文摘要 | chromobox-helicase dna-binding gene (chd1) is the most reliable gene for sex identification in birds. however, the chd1 fragments of some species are difficult to amplify. in this study, we designed a new primer set (intp2/intp8) that targets a conserved region of chd1 gene. firstly, we tested this protocol in oriental skylark (alauda gulgula). pcr amplification produced a single band (259 bp) for males and two bands (259 and 297 bp) for females. we then successfully conducted sex identification in other bird species including tibetan lark (melanocorypha maxima), horned lark (eremophila alpestris), mongolian skylark (melanocorypha mongolica), asian short-toed lark (calandrella cheleensis), humes short-toed lark (calandrella acutirostris), crested lark (galerida cristata), yellow-headed wagtail (motacilla citreola), white wagtail (motacilla alba) and ground tit (pseudopodoces humilis hume). collectively, these findings support that intp2/intp8 primer set can be used to accurately determine sex identity in birds. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biodiversity conservation ; genetics & heredity |
| 研究领域[WOS] | biodiversity & conservation ; genetics & heredity |
| 关键词[WOS] | birds |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000349908000006 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5577]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Hunan Inst Microbiol, Changsha 410009, Hunan, Peoples R China 4.Key Lab Sheep Breed & Biotechnol Xinjiang Bingtua, Shihezi 832000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chu, Hui,Guo, Xinyi,Zeng, Yan,et al. A new primer-pair for sex identification of larks and wagtails[J]. conservation genetics resources,2015,7(1):19-21. |
| APA | Chu, Hui,Guo, Xinyi,Zeng, Yan,Zou, Xiaoyan,Guo, Songchang,&Zhao, Liang.(2015).A new primer-pair for sex identification of larks and wagtails.conservation genetics resources,7(1),19-21. |
| MLA | Chu, Hui,et al."A new primer-pair for sex identification of larks and wagtails".conservation genetics resources 7.1(2015):19-21. |
入库方式： OAI收割
来源：西北高原生物研究所
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