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	一种星斑川鲽精子超低温冷冻保存方法专利 OAI收割专利类型: 发明, 专利号: CN201310213169.0, 申请日期: 2013-09-25, 公开日期: 2013-09-25 刘清华; 李军; 徐世宏; 韩龙江收藏 \| 浏览/下载：33/0 \| 提交时间：2014/08/04 一种星斑川鲽精子超低温冷冻保存方法处理后投入到液氮（‑196℃）所述抗冻液由稀释液其中抗冻剂为终浓度8%的（V/V）DMSO（二甲基亚砜）和终浓度7%的（V/V）Gly（甘油）添加剂：5mM还原型谷胱甘肽其特征在于：将星斑川鲽的精液与抗冻液按体积比1:3的比例混合分装保存抗冻剂和添加剂组成稀释液为100mM蔗糖 50mM牛磺酸在0℃孵育6‑8分钟 100mM KHCO3 10%蛋黄（V/V）。孵育后以分段方式降温处理 25mM NaHCO3
	一种从沙蜇刺丝囊毒素内分离溶血毒素蛋白的方法专利 OAI收割专利类型: 发明, 专利号: CN201110414952.4, 申请日期: 2012-06-27, 公开日期: 2012-06-27 作者: 于华华; 邢荣娥; 李鹏程; 秦玉坤收藏 \| 浏览/下载：38/0 \| 提交时间：2014/08/04 一种从沙蜇刺丝囊毒素中分离溶血毒素蛋白的方法所述洗脱液为含不同浓度NaCl的磷酸缓冲液收集采用含0.2M?NaCl的磷酸缓冲液洗脱下的组分 2)将上述所得洗脱组分经微孔滤膜过滤其特征在于：1)将处理后的沙蜇刺丝囊细胞上清液经微孔滤膜过滤 NaCl浓度分别为0 滤液经凝胶色谱柱Superdex200采用0.15M?NaCl 滤液经含DEAE?Sepharose?Fast?Flow阴离子交换柱 0.1 10～50mM 采用洗脱液以1?3mLmin?1流速进行纯化分离 0.2 pH7.0PBS缓冲液以0.2～1.0mLmin?1流速进行分离纯化收集洗脱液在低温下用3～5K的超滤管进行浓缩 0.3和2M 收集洗脱体积处于60～80mL的洗脱液待用而后在2～6℃下用3～5K的超滤管进行浓缩浓缩后即得到从沙蜇刺丝囊毒素中分离溶血毒素蛋白。
	Design and simulation of CMOS star sensor lens with large relative aperture and wide field (EI CONFERENCE) 会议论文 OAI收割 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011, August 12, 2011 - August 14, 2011, Harbin, China Zhong X.; Jin G. 收藏 \| 浏览/下载：60/0 \| 提交时间：2013/03/25 A 50mm focal length F/1.25 Gaussian type optical system is designed in this paper which has 20field of view. Actual star image adding optical cross-talk effect of CMOS(Complementary Metal Oxide Semiconductor) detector is simulated by non-sequential ray tracing in ZEMAX optical design and analysis software to evaluate its performance. The distortion relative to image spot centriod is introduced and calculated by simulated star images which is less than 0.03% of lens in this paper. Thermal adaptability is discussed by opt-mechanic thermal analysis which shows the RMS spot radius of marginal filed changes less than 0.7m under 10C temperature gradient and changes less than 1m during homogeneous temperature changing from 40C to 40C. The results of analyses and simulations show the design of this lens can achieve the requirement of high precision CMOS star sensor well. 2011 IEEE.