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广州能源研究所 [2]
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期刊论文 [7]
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Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method
期刊论文
OAI收割
JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN, 2021, 卷号: 43, 期号: 1, 页码: 80-87
作者:
Zhao, Cheng
;
Yao, Qing-Song
;
Wang, Can
;
Luo, Mu-Tan
;
Huang, Chao
  |  
收藏
  |  
浏览/下载:38/0
  |  
提交时间:2021/10/27
Microbial lipid
Fluorescent method
Lipid content
Trichosporon dermatis
Lignocellulosic hydrolysates
Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method
期刊论文
OAI收割
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2019, 页码: 9
作者:
Zhao, Cheng
;
Luo, Mu-Tan
;
Huang, Chao
;
Chen, Xue-Fang
;
Xiong, Lian
  |  
收藏
  |  
浏览/下载:22/0
  |  
提交时间:2020/10/29
Fluorescent method
microbial lipid
Nile Red
oleaginous yeasts
食用蔬菜能吸收和积累微塑料
期刊论文
OAI收割
科学通报, 2019, 卷号: 64, 期号: 9, 页码: 928-934
作者:
李连祯
;
周倩
;
尹娜
;
涂晨
;
骆永明
  |  
收藏
  |  
浏览/下载:142/0
  |  
提交时间:2020/06/17
微塑料
生菜
聚苯乙烯微球
吸收
积累
健康风险
microplastics
lettuce
polystyrene microbeads
uptake
accumulation
human health risk
Microplastic (MP, 100 nm-5 mm) may present an attributable risk to ecosystem and human health, and its pollution has become a global environmental concern. Despite a wealth of information on the accumulation of MPs in aquatic species, there is no information on the uptake and accumulation of MPs by higher plants. Terrestrial edible plants are directly exposed to MPs when agricultural soil was applied with organic manure, sewage sludge as fertilizer or plastic mulching. In this paper, the uptake of two sizes of polystyrene (PS) microbeads (0.2 and 1.0 mum) and then their distribution and migration in an edible plant lettuce were firstly investigated based on laboratory experiments. We used fluorescent markers to track PS microbeads in plant tissues and found fluorescence to be a sensitive and reliable detection method. Sections from untreated control lettuce showed no autofluorescence. When roots were treated with fluorescently labeled PS microbeads, the microbeads could be identified by its fluorescence. Our main study investigated the uptake of 0.2 mum beads, as few luminescence signals were observed in lettuce roots for 1.0 mum beads in our experiment. We observed that 0.2 mum fluorescent microbeads were extracellularly trapped in the root cap mucilage (which is a highly hydrated polysaccharide) and a dark green tip (which was typical of lettuce roots exposed to label PS beads) was usually visible to the naked eye. Confocal images revealed that the PS luminescence signals were mainly located in the vascular system and on the cell walls of the cortex tissue of the roots, indicated that the beads passed through the intercellular space via the apoplastic transport system. Once inside the central cylinder, the 0.2 mum PS beads were transferred from the roots to the stems and leaves via the vascular system following the transpiration stream. We also observed that the PS beads adhered to one another and self-assembled systematically into grape-like and (chain) string-like clusters in the intercellular space of the root and stem vascular tissue of lettuce plant. In contrast to the root and stem, PS beads were dispersed in the leaf tissue. Here, for the first time we provide evidence of the adherence, uptake, accumulation, and translocation of submicrometer MPs within an edible plant. Our findings highlight the previously underappreciated human exposure pathway to MPs through the consumption of contaminated crops and emphasize the need for new management strategies to control the release of MPs waste products into the terrestrial environment. Ultimately, the potential impacts of low range sized MPs on food safety of crop plants and human health need to be urgently considered.
卤代醌/过氧化氢体系产生羟基自由基的荧光监测、反应动力学及分子机理研究
学位论文
OAI收割
博士, 北京: 中国科学院研究生院, 2015
作者:
任福荣
收藏
  |  
浏览/下载:216/0
  |  
提交时间:2016/08/18
卤代醌
氯代萘醌
荧光法
羟基自由基
动力学,Halogenated Quinones
Chlorinated 1
4-Naphthoquinone
Fluorescent method
Hydroxyl Radical
Kinetics
Transition from Eu3+ to Eu2+ in SiO2 Matrix Prepared by Sol-Gel
期刊论文
OAI收割
journal of nanoscience and nanotechnology, 2014, 卷号: 14, 期号: 5, 页码: 3642-3647
作者:
Zhang, Dekai
;
Hu, Xiaoyun
;
Jing, Guangyin
;
Liu, Enzhou
;
Fan, Jun
收藏
  |  
浏览/下载:37/0
  |  
提交时间:2015/03/26
Activated Silica
Fluorescent Materials
Al3+ and Eu2+ Doping
Sol-Gel Method
Monodisperse lanthanide oxyfluorides LnOF (Ln = Y, La, Pr-Tm): morphology controlled synthesis, up-conversion luminescence and in vitro cell imaging
期刊论文
OAI收割
nanoscale, 2014, 卷号: 6, 期号: 12, 页码: 6763-6771
Zhang, Yang
;
Li, Xuejiao
;
Hou, Zhiyao
;
Lin,Jun
收藏
  |  
浏览/下载:32/0
  |  
提交时间:2015/03/27
TUNABLE EMISSION PROPERTIES
RARE-EARTH FLUORIDE
GEL PECHINI METHOD
DRUG-DELIVERY
HYDROTHERMAL SYNTHESIS
COLLOIDAL SPHERES
FLUORESCENT NANOPARTICLES
COORDINATION CHEMISTRY
HOLLOW SPHERES
LARGE-SCALE
Dummy Molecularly Imprinted Polymers-Capped CdTe Quantum Dots for the Fluorescent Sensing of 2,4,6-Trinitrotoluene
期刊论文
OAI收割
ACS APPLIED MATERIALS & INTERFACES, 2013, 卷号: 5, 期号: 16, 页码: 8146-8154
作者:
Xu, Shoufang
;
Lu, Hongzhi
;
Li, Jinhua
;
Song, Xingliang
;
Wang, Aixiang
收藏
  |  
浏览/下载:44/0
  |  
提交时间:2014/07/08
quantum dots
molecularly imprinted polymers
dummy template
fluorescent detection
seed-growth method
2
4
6-trinitrotoluene
Transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) by agitation with glass beads
期刊论文
OAI收割
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2010, 卷号: 26, 期号: 9, 页码: 1653-1657
作者:
Cui, Yulin
;
Wang, Jinfeng
;
Jiang, Peng
;
Bian, Shuguang
;
Qin, Song
  |  
收藏
  |  
浏览/下载:21/0
  |  
提交时间:2010/12/24
Glass-bead Method
Green Fluorescent Protein
Platymonas Subcordiformis
Protoplast
Transgenosis