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Glycoengineering and glycosite-specific labeling of serum IgGs from various species 期刊论文  OAI收割
CARBOHYDRATE RESEARCH, 2017, 卷号: 446, 页码: 32-39
作者:  
Guo, Weilai;  Tang, Feng
  |  收藏  |  浏览/下载:68/0  |  提交时间:2019/01/08
A study of interaction between FITC- labeled antibody and optical fiber surface (EI CONFERENCE) 会议论文  OAI收割
ICO20: Materials and Nanostructures, August 21, 2005 - August 26, 2005, Changchun, China
作者:  
Wang X.;  Wang X.;  Wang X.
收藏  |  浏览/下载:38/0  |  提交时间:2013/03/25
The immobilization of antibodies on solid surface has been widely studied in elucidating immunoassay and in developing immunosensor. In this paper  FITC-labeled goat anti-human IgG were covalently immobilized on 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify the distal end of a fiber-optic probe. The higher density and stability of FITC-labeled goat anti-human IgG could be obtained on the fiber surface modified with APTES-Glu than that on the non-modified fiber surface by evanescent excited fluorescence spectroscopy. Furthermore  red shift of the FITC fluorescence dye emission peak was observed on different fiber surface  exhibiting different interaction between FITC- labeled antibody and surface. Finally  under these conditions antigen-antibody reaction was investigated as an immunosensor. Detection limit of the evanescent wave optic-fiber biosensor is 10ng/ml.