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Chinese Academy of Sciences Institutional Repositories Grid
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CAS IR Grid
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长春光学精密机械与物... [1]
上海药物研究所 [1]
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OAI收割 [2]
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会议论文 [1]
期刊论文 [1]
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2017 [1]
2006 [1]
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Glycoengineering and glycosite-specific labeling of serum IgGs from various species
期刊论文
OAI收割
CARBOHYDRATE RESEARCH, 2017, 卷号: 446, 页码: 32-39
作者:
Guo, Weilai
;
Tang, Feng
  |  
收藏
  |  
浏览/下载:68/0
  |  
提交时间:2019/01/08
Chemoenzymatic glycoengineering
Serum IgG
Glycosite-specific IgG labeling
Rabbit IgG
Mouse IgG
Goat IgG
A study of interaction between FITC- labeled antibody and optical fiber surface (EI CONFERENCE)
会议论文
OAI收割
ICO20: Materials and Nanostructures, August 21, 2005 - August 26, 2005, Changchun, China
作者:
Wang X.
;
Wang X.
;
Wang X.
收藏
  |  
浏览/下载:38/0
  |  
提交时间:2013/03/25
The immobilization of antibodies on solid surface has been widely studied in elucidating immunoassay and in developing immunosensor. In this paper
FITC-labeled goat anti-human IgG were covalently immobilized on 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify the distal end of a fiber-optic probe. The higher density and stability of FITC-labeled goat anti-human IgG could be obtained on the fiber surface modified with APTES-Glu than that on the non-modified fiber surface by evanescent excited fluorescence spectroscopy. Furthermore
red shift of the FITC fluorescence dye emission peak was observed on different fiber surface
exhibiting different interaction between FITC- labeled antibody and surface. Finally
under these conditions antigen-antibody reaction was investigated as an immunosensor. Detection limit of the evanescent wave optic-fiber biosensor is 10ng/ml.