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	Protein A for human IgG oriented immobilization on silicon surface for an imaging ellipsometry biosensor (EI CONFERENCE) 会议论文 OAI收割 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009, September 2, 2009 - September 6, 2009, Minneapolis, MN, United states 作者: Chen B.; Chen B. 收藏 \| 浏览/下载：19/0 \| 提交时间：2013/03/25 Imaging ellipsometry biosensor was developed on the basement of imaging ellipsometry for thin-film and surface characterization. Using protein A to immobilize Human IgG on silicon surface for the imaging ellipsometry biosensor immunoassay was studied. As a result The Human IgG immobilized on silicon surface by protein A bound much more polyclonal antibody than that on hydrophobic surface directly which indicated that protein A could be used to immobilize Human IgG molecules in a highly oriented manner and Human IgG molecule could maintain its native configuration well on the silicon surface. Furthermore the absorption quantity of Protein A affecting on interaction of Human IgG and antibody molecules was investigated. The surface concentration of Human IgG and antibody molecules was enhanced as well as the increasing of the absorption quantity of Protein A but there are no obvious increase when the ratio of surface concentration of Protein A layer to that of saturated adsorption is larger than 80%. However even decrease slightly. 2009 IEEE.
	Fast purification of trace vitellogenin from Chinese rare minnow using protein A-immobilized antibody 期刊论文 OAI收割 ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 卷号: 390, 期号: 8, 页码: 2151-2157 Song, Maoyong; Lv, Xuefei; Wang, Hailin; Jiang, Guibin 收藏 \| 浏览/下载：19/0 \| 提交时间：2015/08/20 vitellogenin (Vtg) affinity purification immobilized antibody
	A study of interaction between FITC- labeled antibody and optical fiber surface (EI CONFERENCE) 会议论文 OAI收割 ICO20: Materials and Nanostructures, August 21, 2005 - August 26, 2005, Changchun, China 作者: Wang X.; Wang X.; Wang X. 收藏 \| 浏览/下载：40/0 \| 提交时间：2013/03/25 The immobilization of antibodies on solid surface has been widely studied in elucidating immunoassay and in developing immunosensor. In this paper FITC-labeled goat anti-human IgG were covalently immobilized on 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify the distal end of a fiber-optic probe. The higher density and stability of FITC-labeled goat anti-human IgG could be obtained on the fiber surface modified with APTES-Glu than that on the non-modified fiber surface by evanescent excited fluorescence spectroscopy. Furthermore red shift of the FITC fluorescence dye emission peak was observed on different fiber surface exhibiting different interaction between FITC- labeled antibody and surface. Finally under these conditions antigen-antibody reaction was investigated as an immunosensor. Detection limit of the evanescent wave optic-fiber biosensor is 10ng/ml.