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CAS IR Grid
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长春光学精密机械与物... [2]
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OAI收割 [2]
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会议论文 [2]
发表日期
2009 [2]
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Protein A for human IgG oriented immobilization on silicon surface for an imaging ellipsometry biosensor (EI CONFERENCE)
会议论文
OAI收割
31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009, September 2, 2009 - September 6, 2009, Minneapolis, MN, United states
作者:
Chen B.
;
Chen B.
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浏览/下载:14/0
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提交时间:2013/03/25
Imaging ellipsometry biosensor was developed on the basement of imaging ellipsometry for thin-film and surface characterization. Using protein A to immobilize Human IgG on silicon surface for the imaging ellipsometry biosensor immunoassay was studied. As a result
The Human IgG immobilized on silicon surface by protein A bound much more polyclonal antibody than that on hydrophobic surface directly
which indicated that protein A could be used to immobilize Human IgG molecules in a highly oriented manner and Human IgG molecule could maintain its native configuration well on the silicon surface. Furthermore
the absorption quantity of Protein A affecting on interaction of Human IgG and antibody molecules was investigated. The surface concentration of Human IgG and antibody molecules was enhanced as well as the increasing of the absorption quantity of Protein A
but there are no obvious increase when the ratio of surface concentration of Protein A layer to that of saturated adsorption is larger than 80%. However
even decrease slightly. 2009 IEEE.
Protein A for Human IgG Oriented Immobilization on Silicon Surface for an Imaging Ellipsometry biosensor
会议论文
OAI收割
2009
作者:
Chen B.
;
Chen B.
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浏览/下载:7/0
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提交时间:2013/03/28