机构

• 长春光学精密机械与物... [2]

采集方式

• OAI收割 [2]

内容类型

• 会议论文 [2]

发表日期

• 2009 [2]

学科主题

筛选

浏览/检索结果: 共2条，第1-2条 帮助 限定条件 专题：长春光学精密机械与物理研究所    第一署名单位    第一作者单位    通讯作者单位     条数/页： 5101520253035404550556065707580859095100 排序方式： 请选择提交时间升序提交时间降序发表日期升序发表日期降序题名升序题名降序作者升序作者降序 Protein A for human IgG oriented immobilization on silicon surface for an imaging ellipsometry biosensor (EI CONFERENCE) 会议论文  OAI收割 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009, September 2, 2009 - September 6, 2009, Minneapolis, MN, United states 作者:  Chen B.;  Chen B. 收藏  |  浏览/下载：14/0  |  提交时间：2013/03/25 Imaging ellipsometry biosensor was developed on the basement of imaging ellipsometry for thin-film and surface characterization. Using protein A to immobilize Human IgG on silicon surface for the imaging ellipsometry biosensor immunoassay was studied. As a result  The Human IgG immobilized on silicon surface by protein A bound much more polyclonal antibody than that on hydrophobic surface directly  which indicated that protein A could be used to immobilize Human IgG molecules in a highly oriented manner and Human IgG molecule could maintain its native configuration well on the silicon surface. Furthermore  the absorption quantity of Protein A affecting on interaction of Human IgG and antibody molecules was investigated. The surface concentration of Human IgG and antibody molecules was enhanced as well as the increasing of the absorption quantity of Protein A  but there are no obvious increase when the ratio of surface concentration of Protein A layer to that of saturated adsorption is larger than 80%. However  even decrease slightly. 2009 IEEE.   Protein A for Human IgG Oriented Immobilization on Silicon Surface for an Imaging Ellipsometry biosensor 会议论文  OAI收割 2009 作者:  Chen B.;  Chen B. 收藏  |  浏览/下载：7/0  |  提交时间：2013/03/28