Selective pattern of cancer cell accumulation and growth using UV modulating printing of hydrogels
文献类型：期刊论文
| 作者 | Yang WG(杨文广); Yu HB(于海波) ; Wei FN(魏发南); Li GX(李恭新); Wang YC(王越超); Liu LQ(刘连庆)
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| 刊名 | Biomedical Microdevices
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| 出版日期 | 2015 |
| 卷号 | 17期号:6页码:1-8 |
| 关键词 | Cell pattern Microfabrication Bioengineering Bioprinting |
| ISSN号 | 1387-2176 |
| 产权排序 | 1 |
| 通讯作者 | 于海波 ; 刘连庆 |
| 中文摘要 | Fabrication of extracellular microenvironment for cancer cell growth in vitro is an indispensable technique to precisely control the cell spatial arrangement and proliferation for cell-behavior research. Current micropatterning methods usually require relatively complicated operations, which makes it difficult to investigate the effects of different cell growth patterns. This manuscript proposes a DMD-based projection technique to quickly pattern a poly(ethylene) glycol diacrylate (PEGDA)-based hydrogel on a common glass substrate. Using this method, we can effectively control the growth patterns of cells. Compared with these traditional methods which employ digital dynamic mask, polymerization of PEGDA solution can be used to create arbitrary shaped microstructures with high efficiency, flexibility and repeatability. The duration of UV exposure is less than 10 s through controlling the projected illumination pattern. The ability of patterned PEGDA-coated film to hinder cell adhesion makes it possible to control area over which cells attach. In our experiments, we take advantage of the blank area to pattern cells, which allows cells to grow in various pre-designed shapes and sizes. And the patterning cells have a high viability after culturing for several days. Interestingly, we found that the restricted space could stiffen and strengthen the cells. These results indicate that cells and extracellular microenvironment can influence each other. © 2015, Springer Science+Business Media New York. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Biomedical ; Nanoscience & Nanotechnology |
| 研究领域[WOS] | Engineering ; Science & Technology - Other Topics |
| 关键词[WOS] | ATOMIC-FORCE MICROSCOPY ; SELF-ASSEMBLED MONOLAYERS ; MECHANICAL-PROPERTIES ; BREAST-CANCER ; LIVING CELLS ; ADHESION ; LITHOGRAPHY ; DIFFERENTIATION ; MICROSTRUCTURES ; PEPTIDES |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000365704800003 |
| 源URL | [http://ir.sia.cn/handle/173321/17140]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 推荐引用方式 GB/T 7714 | Yang WG,Yu HB,Wei FN,et al. Selective pattern of cancer cell accumulation and growth using UV modulating printing of hydrogels[J]. Biomedical Microdevices,2015,17(6):1-8. |
| APA | Yang WG,Yu HB,Wei FN,Li GX,Wang YC,&Liu LQ.(2015).Selective pattern of cancer cell accumulation and growth using UV modulating printing of hydrogels.Biomedical Microdevices,17(6),1-8. |
| MLA | Yang WG,et al."Selective pattern of cancer cell accumulation and growth using UV modulating printing of hydrogels".Biomedical Microdevices 17.6(2015):1-8. |
入库方式： OAI收割
来源：沈阳自动化研究所
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