Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties
文献类型：期刊论文
| 作者 | Liu, Miao; Zheng, Mingjing; Xu, Hanying; Liu LQ(刘连庆) ; Li, Yanchun; Xiao, Wei; Li JC(李建春); Ma, Enlong
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| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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| 出版日期 | 2015 |
| 卷号 | 468期号:1-2页码:214－220 |
| 关键词 | Idiopathic pulmonary fibrosis (IPF) Cyto-biophysical properties Atomic force microscopy (AFM) Young's modulus A549 cells Salvianolic acid B (SalB) |
| ISSN号 | 0006-291X |
| 产权排序 | 2 |
| 通讯作者 | 李建春 ; Ma, Enlong |
| 中文摘要 | Various methods have been used to evaluate anti-fibrotic activity of drugs. However, most of them are complicated, labor-intensive and lack of efficiency. This study was intended to develop a rapid method for anti-fibrotic drugs screening based on biophysical properties. A549 cells in vitro were stimulated with transforming growth factor-beta 1 (TGF-beta 1), and fibrogenesis was confirmed by conventional immunological assays. Meanwhile, the alterations of cyto-biophysical properties including morphology, roughness and stiffness were measured utilizing atomic force microscopy (AFM). It was found that fibrogenesis was accompanied with changes of cellular biophysical properties. TGF-beta 1-stimulated A549 cells became remarkably longer, rougher and stiffer than the control. Then, the effect of N-acetyl-L-cysteine (NAC) as a positive drug on ameliorating fibrogenesis in TGF-beta 1-stimulated A549 cells was verified respectively by immunological and biophysical markers. The result of Principal Component Analysis showed that stiffness was a leading index among all biophysical markers during fibrogenesis. Salvianolic acid B (SalB), a natural anti-oxidant, was detected by AFM to protect TGF-beta 1-stimulated A549 cells against stiffening. Then, SalB treatment was provided in preventive mode on a rat model of bleomycin (BLM) -induced pulmonary fibrosis. The results showed that SalB treatment significantly ameliorated BLM-induced histological alterations, blocked collagen accumulations and reduced alpha-SMA expression in lung tissues. All these results revealed the anti-pulmonary fibrotic activity of SalB. Detection of cyto-biophysical properties were therefore recommended as a rapid method for anti-pulmonary fibrotic drugs screening. (C) 2015 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ATOMIC-FORCE MICROSCOPY ; INTERMEDIATE-FILAMENTS ; CELLS ; FIBROSIS ; BIOMECHANICS ; CARCINOMA ; VIMENTIN ; CANCER ; AFM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000366240800035 |
| 源URL | [http://ir.sia.cn/handle/173321/17577]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 推荐引用方式 GB/T 7714 | Liu, Miao,Zheng, Mingjing,Xu, Hanying,et al. Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,468(1-2):214－220. |
| APA | Liu, Miao.,Zheng, Mingjing.,Xu, Hanying.,Liu LQ.,Li, Yanchun.,...&Ma, Enlong.(2015).Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,468(1-2),214－220. |
| MLA | Liu, Miao,et al."Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 468.1-2(2015):214－220. |
入库方式： OAI收割
来源：沈阳自动化研究所
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