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3D bioprinting of BMSC-laden methacrylamide gelatin scaffolds with CBD-BMP2-collagen microfibers
文献类型：期刊论文
| 作者 | Du, Mingchun; Chen B(陈冰); Meng QY(孟庆圆); Liu SM(刘素梅); Zheng XF(郑雄飞) ; Zhang C(张诚); Wang HR(王赫然); Li HY(李洪谊); Wang N(王诺); Dai JW(戴建武)
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| 刊名 | BIOFABRICATION
 |
| 出版日期 | 2015 |
| 卷号 | 7期号:4页码:1-10 |
| 关键词 | 3D bioprinting BMSC methacrylamide gelatin scaffold CBD-BMP2-collagen microfiber osteogenic differentiation |
| ISSN号 | 1758-5082 |
| 产权排序 | 3 |
| 通讯作者 | Du, Mingchun |
| 中文摘要 | Three-dimensional (3D) bioprinting combines biomaterials, cells and functional components into complex living tissues. Herein, we assembled function-control modules into cell-laden scaffolds using 3D bioprinting. A customized 3D printer was able to tune the microstructure of printed bone mesenchymal stem cell (BMSC)-laden methacrylamide gelatin scaffolds at the micrometer scale. For example, the pore size was adjusted to 282 +/- 32 mu m and 363 +/- 60 mu m. To match the requirements of the printing nozzle, collagen microfibers with a length of 22 +/- 13 mu m were prepared with a highspeed crusher. Collagen microfibers bound bone morphogenetic protein 2 (BMP2) with a collagen binding domain (CBD) as differentiation-control module, from which BMP2 was able to be controllably released. The differentiation behaviors of BMSCs in the printed scaffolds were compared in three microenvironments: samples without CBD-BMP2-collagen microfibers in the growth medium, samples without microfibers in the osteogenic medium and samples with microfibers in the growth medium. The results indicated that BMSCs showed high cell viability (>90%) during printing; CBD-BMP2-collagen microfibers induced BMSC differentiation into osteocytes within 14 days more efficiently than the osteogenic medium. Our studies suggest that these function-control modules are attractive biomaterials and have potential applications in 3D bioprinting. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Biomedical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Engineering ; Materials Science |
| 关键词[WOS] | MESENCHYMAL STEM-CELLS ; BONE MORPHOGENETIC PROTEIN-2 ; FIBROBLAST-GROWTH-FACTOR ; CROSS-LINKING HEPARIN ; COLLAGEN SCAFFOLDS ; EXTRACELLULAR-MATRIX ; ENGINEERED CARTILAGE ; CHONDROITIN SULFATE ; TISSUE ; REGENERATION |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000366896900004 |
| 源URL | [http://ir.sia.cn/handle/173321/17578]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 推荐引用方式 GB/T 7714 | Du, Mingchun,Chen B,Meng QY,et al. 3D bioprinting of BMSC-laden methacrylamide gelatin scaffolds with CBD-BMP2-collagen microfibers[J]. BIOFABRICATION,2015,7(4):1-10. |
| APA | Du, Mingchun.,Chen B.,Meng QY.,Liu SM.,Zheng XF.,...&Dai JW.(2015).3D bioprinting of BMSC-laden methacrylamide gelatin scaffolds with CBD-BMP2-collagen microfibers.BIOFABRICATION,7(4),1-10. |
| MLA | Du, Mingchun,et al."3D bioprinting of BMSC-laden methacrylamide gelatin scaffolds with CBD-BMP2-collagen microfibers".BIOFABRICATION 7.4(2015):1-10. |
入库方式： OAI收割
来源：沈阳自动化研究所
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