Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening
文献类型：期刊论文
| 作者 | Ma JY(马静云); Zhang, Xu; Liu Y(刘扬); Yu HB(于海波) ; Liu LQ(刘连庆); Shi Y(石杨); Li YF(李艳峰); Qin JH(秦建华)
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| 刊名 | Biotechnology Journal
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| 出版日期 | 2016 |
| 卷号 | 11期号:1页码:127-134 |
| 关键词 | Drug screening Hypoxia-inducible factors (HIFs) Micro-patterning Multicellular spheroids Phenotype transition |
| ISSN号 | 1860-6768 |
| 产权排序 | 1 |
| 通讯作者 | 秦建华 |
| 中文摘要 | 3D multicellular spheroid models are of great value in the investigation of tumor biology and tumor responses to chemotherapy and radiation. To establish a mimicking tumor microenvironment in vitro, we developed a straightforward method by patterning hypoxic multicellular spheroids in a 3D matrix. The efficacy of this approach was evaluated by characterizing spheroid formation, invasive capability and phenotypic transition in aggressive human glioma cells. We observed enhanced cell proliferation, spheroid formation and invasive capability in U87 glioma cells transfected with hypoxia-inducible factors (HIFs) compared with non-treated cells. We also demonstrated that the overexpression of HIFs in hypoxic glioma cells may promote cell migration by epithelial-mesenchymal transition within the 3D matrix. Compared with conventional 3D culturing techniques, the simple operation, rapid prototyping, low cost and high throughput format of the micro-patterning method facilitates the characterization of cell proliferation, migration, phenotypic function and drug evaluation in physiologically relevant 3D microenvironments. This in vitro 3D system can recapitulate the physiologically relevant tumor microenvironment and is a promising method for 3D anti-tumor drug screening and the identification of novel targets for tumor invasion and angiogenesis. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | CELL-CULTURE SYSTEMS ; IN-VITRO ; MESENCHYMAL TRANSITION ; CANCER ; MODELS ; GROWTH ; IDENTIFICATION ; DISCOVERY ; INVASION ; 3-D |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000370002300012 |
| 源URL | [http://ir.sia.cn/handle/173321/17615]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 推荐引用方式 GB/T 7714 | Ma JY,Zhang, Xu,Liu Y,et al. Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening[J]. Biotechnology Journal,2016,11(1):127-134. |
| APA | Ma JY.,Zhang, Xu.,Liu Y.,Yu HB.,Liu LQ.,...&Qin JH.(2016).Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening.Biotechnology Journal,11(1),127-134. |
| MLA | Ma JY,et al."Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening".Biotechnology Journal 11.1(2016):127-134. |
入库方式： OAI收割
来源：沈阳自动化研究所
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