Down-regulation of PKC zeta expression inhibits chemotaxis signal transduction in human lung cancer cells
文献类型：期刊论文
| 作者 | Liu, Y; Wang, B; Wang, JN; Wan, WZ; Sun, RH; Zhao, YL; Zhang, N; Zhao YL(赵宇亮) |
| 刊名 | LUNG CANCER
 |
| 出版日期 | 2009 |
| 卷号 | 63期号:2页码:#REF! |
| 关键词 | NSCLC PKC zeta Chemotaxis EGFR Metastasis Pseudosubstrate |
| 通讯作者 | Zhang, N (reprint author), Peking Univ, Dept Biol Chem, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China. |
| 英文摘要 | Metastasis is the major cause of mortality in lung cancer. Chemotaxis plays a vital role in cancer cell metastasis. In the current study, we reported that epidermal growth factor (EGF) induced a robust chemotaxis of A549 and H1299 cells, two representative human non-small cell lung cancer (NSCLC) cells. Chelerythrine chloride, an inhibitor of all protein kinase C (PKC) isozymes, significantly reduced the chemotactic capacity of NSCLC cells while inhibitors of classical or novel PKC isozymes, such as 666976, calphostin C, or Go6850, showed no effect, which suggested that atypical PKC might be involved in the chemotactic process of NSCLC cells. EGF-elicited translocation and phosphorylation of atypical PKC, indicating that EGF could activate PKC zeta. Treatment with a PKC zeta specific inhibitor, a myristoylated pseudosubstrate, blocked the chemotaxis in a dose-dependent manner, further confirming that atypical PKCI; was required for NSCLC chemotaxis. Mechanistic studies suggested that PKC zeta; was regulated by phosphatidylinositol 3 kinase (PI3K)/Akt. Furthermore, PKC zeta-mediated chemotaxis by regulating actin polymerization and cell adhesion. Taken together, our study suggested that PKC zeta was required in NSCLC cell chemotaxis, thus could be used as a target to develop anti-lung cancer metastasis therapies. (C) 2008 Published by Elsevier Ireland Ltd. |
| 学科主题 | Oncology; Respiratory System |
| 类目[WOS] | Oncology ; Respiratory System |
| 收录类别 | SCI |
| WOS记录号 | WOS:000263020700008 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/226963]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Liu, Y,Wang, B,Wang, JN,et al. Down-regulation of PKC zeta expression inhibits chemotaxis signal transduction in human lung cancer cells[J]. LUNG CANCER,2009,63(2):#REF!. |
| APA | Liu, Y.,Wang, B.,Wang, JN.,Wan, WZ.,Sun, RH.,...&赵宇亮.(2009).Down-regulation of PKC zeta expression inhibits chemotaxis signal transduction in human lung cancer cells.LUNG CANCER,63(2),#REF!. |
| MLA | Liu, Y,et al."Down-regulation of PKC zeta expression inhibits chemotaxis signal transduction in human lung cancer cells".LUNG CANCER 63.2(2009):#REF!. |
入库方式： OAI收割
来源：高能物理研究所
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