基于光诱导介电泳机械力的免标记细胞电特性获取方法
文献类型：专利
| 作者 | 刘连庆 ; 梁文峰; 李文荣; 张伟京; 肖秀斌
|
| 发表日期 | 2016-04-13 |
| 专利国别 | 中国 |
| 专利号 | CN105486867A |
| 专利类型 | 发明 |
| 产权排序 | 1 |
| 权利人 | 中国科学院沈阳自动化研究所 |
| 其他题名 | Method for acquiring label-free cells electrical characteristics based on light-induced dielectrophoresis mechanical force |
| 是否PCT专利 | 否 |
| 中文摘要 | 本发明涉及基于光诱导介电泳机械力的免标记细胞电特性获取方法，包括：将入射光投影到光电子镊芯片下表面；信号发生器发出交流电压的频率，作用于光电子镊芯片的导电层；细胞开始远离入射光运动；增加外加交流电压的频率，当某细胞静止时，此时的频率为该细胞的交越频率；继续增加外加交流电压的频率，该细胞朝入射光处运动；当所有细胞均进入入射光范围内，关闭入射光及外加交流电压；得到各细胞的交越频率和直径的关系；根据细胞膜电容与细胞直径的关系，得到各细胞膜电容。本发明无需制备特异性结构的金属微电极；可实时获取单个或多个细胞的电特性参数；无需利用抗体对细胞进行标识，具有自动化、批量化及吞吐量高的优点。 |
| 英文摘要 | The invention relates to a method for acquiring label-free cells electrical characteristics based on light-induced dielectrophoresis mechanical force. The method comprises the following steps: incident light is projected to a lower surface of a photoelectron tweezer chip; frequency of alternating voltage emitted by a signal-generator is affected on a conducting layer of the photoelectron tweezer chip; cells move far from the incident light; frequency of applied alternating voltage can be increased, when certain cell is stationary, the frequency is a cross-over frequency of the cell; frequency of the applied alternating voltage is continuously increased, the cell moves towards to the incident light; when all the cells move in an incident light scope, the incident light and the applied alternating voltage are closed; a relation between the cross-over frequency and a diameter of each cell can be obtained; and capacitance of each cell membrane can be obtained according to the relation between the cell membrane capacitance and cell diameter. The preparation of metal microelectrodes with a specific structure is not required; the electrical characteristic parameter of single cell or many cells can be obtained with real time; an antibody is not required for being used for identification of the cells, and the method has the advantages of automation, mass production and high throughput capacity. |
| 申请日期 | 2014-09-19 |
| 语种 | 中文 |
| 专利申请号 | CN201410482823.2 |
| 专利代理 | 沈阳科苑专利商标代理有限公司 21002 |
| 源URL | [http://ir.sia.cn/handle/173321/18568]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 推荐引用方式 GB/T 7714 | 刘连庆,梁文峰,李文荣,等. 基于光诱导介电泳机械力的免标记细胞电特性获取方法. CN105486867A. 2016-04-13. |
入库方式： OAI收割
来源：沈阳自动化研究所
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