Fully Automated Quantification of Insulin Concentration Using a Microfluidic-Based Chemiluminescence Immunoassay
文献类型：期刊论文
| 作者 | Yao P(姚萍); Liu Z(刘柱) ; Tung, Steve; Dong ZL(董再励); Liu LQ(刘连庆)
|
| 刊名 | Journal of Laboratory Automation
 |
| 出版日期 | 2016 |
| 卷号 | 21期号:3页码:387-393 |
| 关键词 | microfluidics point-of-care testing (POCT) rapid determination insulin, chemiluminescence |
| ISSN号 | 2211-0682 |
| 产权排序 | 1 |
| 通讯作者 | 姚萍 |
| 中文摘要 | A fully automated microfluidic-based detection system for the rapid determination of insulin concentration through a chemiluminescence immunoassay has been developed. The microfluidic chip used in the system is a double-layered polydimethylsiloxane device embedded with interconnecting micropumps, microvalves, and a micromixer. At a high injection rate of the developing solution, the chemiluminescence signal can be excited and measured within a short period of time. The integral value of the chemiluminescence light signal is used to determine the insulin concentration of the samples, and the results indicate that the measurement is accurate in the range from 1.5 pM to 391 pM. The entire chemiluminescence assay can be completed in less than 10 min. The fully automated microfluidic-based insulin detection system provides a useful platform for rapid determination of insulin in clinical diagnostics for diabetes, which is expected to become increasingly important for future point-of-care applications. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | TELEMEDICINE ; SYSTEM |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000377095200005 |
| 源URL | [http://ir.sia.cn/handle/173321/18682]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 推荐引用方式 GB/T 7714 | Yao P,Liu Z,Tung, Steve,et al. Fully Automated Quantification of Insulin Concentration Using a Microfluidic-Based Chemiluminescence Immunoassay[J]. Journal of Laboratory Automation,2016,21(3):387-393. |
| APA | Yao P,Liu Z,Tung, Steve,Dong ZL,&Liu LQ.(2016).Fully Automated Quantification of Insulin Concentration Using a Microfluidic-Based Chemiluminescence Immunoassay.Journal of Laboratory Automation,21(3),387-393. |
| MLA | Yao P,et al."Fully Automated Quantification of Insulin Concentration Using a Microfluidic-Based Chemiluminescence Immunoassay".Journal of Laboratory Automation 21.3(2016):387-393. |
入库方式： OAI收割
来源：沈阳自动化研究所
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