A novel approach for extracting viscoelastic parameters of living cells through combination of inverse finite element simulation and Atomic Force Microscopy
文献类型：期刊论文
| 作者 | Wei FN(魏发南); Yang, Haitao; Liu LQ(刘连庆) ; Li GY(李广勇)
|
| 刊名 | Computer Methods in Biomechanics and Biomedical Engineering
 |
| 出版日期 | 2017 |
| 卷号 | 20期号:4页码:373–384 |
| 关键词 | Viscoelasticity Atomic Force Microscopy finite element simulation |
| ISSN号 | 1025-5842 |
| 产权排序 | 1 |
| 通讯作者 | 刘连庆 ; 李广勇 |
| 中文摘要 | Dynamic mechanical behaviour of living cells has been described by viscoelasticity. However, quantitation of the viscoelastic parameters for living cells is far from sophisticated. In this paper, combining inverse finite element (FE) simulation with Atomic Force Microscope characterization, we attempt to develop a new method to evaluate and acquire trustworthy viscoelastic index of living cells. First, influence of the experiment parameters on stress relaxation process is assessed using FE simulation. As suggested by the simulations, cell height has negligible impact on shape of the force–time curve, i.e. the characteristic relaxation time; and the effect originates from substrate can be totally eliminated when stiff substrate (Young’s modulus larger than 3 GPa) is used. Then, so as to develop an effective optimization strategy for the inverse FE simulation, the parameters sensitivity evaluation is performed for Young’s modulus, Poisson’s ratio, and characteristic relaxation time. With the experiment data obtained through typical stress relaxation measurement, viscoelastic parameters are extracted through the inverse FE simulation by comparing the simulation results and experimental measurements. Finally, reliability of the acquired mechanical parameters is verified with different load experiments performed on the same cell. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Computer Science, Interdisciplinary Applications ; Engineering, Biomedical |
| 研究领域[WOS] | Computer Science ; Engineering |
| 关键词[WOS] | DEPTH-SENSING INDENTATION ; ELASTIC-MODULUS ; SOFT-TISSUES ; NANOINDENTATION ; MODEL ; MECHANICS ; AFM |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000395117200004 |
| 源URL | [http://ir.sia.cn/handle/173321/19198]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 推荐引用方式 GB/T 7714 | Wei FN,Yang, Haitao,Liu LQ,et al. A novel approach for extracting viscoelastic parameters of living cells through combination of inverse finite element simulation and Atomic Force Microscopy[J]. Computer Methods in Biomechanics and Biomedical Engineering,2017,20(4):373–384. |
| APA | Wei FN,Yang, Haitao,Liu LQ,&Li GY.(2017).A novel approach for extracting viscoelastic parameters of living cells through combination of inverse finite element simulation and Atomic Force Microscopy.Computer Methods in Biomechanics and Biomedical Engineering,20(4),373–384. |
| MLA | Wei FN,et al."A novel approach for extracting viscoelastic parameters of living cells through combination of inverse finite element simulation and Atomic Force Microscopy".Computer Methods in Biomechanics and Biomedical Engineering 20.4(2017):373–384. |
入库方式： OAI收割
来源：沈阳自动化研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。