Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice
文献类型：期刊论文
| 作者 | Gu, Rui; Zhang, Xu; Zhang, Ge; Tao, Tingting; Yu HB(于海波) ; Liu LQ(刘连庆); Dou Y(窦颖); Li AP(李爱萍); Qin JH(秦建华)
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| 刊名 | Neurochemical Research
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| 出版日期 | 2017 |
| 卷号 | 42期号:5页码:1478-1487 |
| 关键词 | Microfluidic device Microglia Glioma Epithelial-mesenchymal transition Phagocytosis Migration |
| 产权排序 | 5 |
| 通讯作者 | 李爱萍 ; 秦建华 |
| 中文摘要 | It has been proven that microglia are involved in both early and late stages of glioma progression and contribute substantially to the tumor mass of gliomas. Because no appropriate in vitro or in vivo investigative approach is available, the dynamic interaction between microglia and gliomas during tumor formation remains unclear. In this study, three types of microfluidic assay were developed to examine the outcomes of the dynamic interaction between microglia and gliomas. Co-migration assay and two-dimensional cell co-culture assay have been used to show that microglial BV-2 cells migrate toward C6 glioma cells and inhibit tumor growth during the early stage of tumorigenesis. However, in three-dimensional cell spheres (three-dimensional cell co-culture assay) that contain a large amount of glioma cells, mimicking the late stage of glioma growth, the phagocytosis of microglia was suppressed, which suggests that glioma cells could reeducate classically activated microglia into a tumor-promoting state at some point during tumor progression. Notably, we found that microglia could contribute to tumor invasion and acquisition of the epithelial-mesenchymal transition phenotype in the glioma microenvironment during the early stage and the late stage of tumor progression. In conclusion, we have developed a potential quantitative method for in vitro study of glioma immunity and provided evidence for the duality of glioma-associated microglia. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000399906000021 |
| 源URL | [http://ir.sia.cn/handle/173321/20235]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 推荐引用方式 GB/T 7714 | Gu, Rui,Zhang, Xu,Zhang, Ge,et al. Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice[J]. Neurochemical Research,2017,42(5):1478-1487. |
| APA | Gu, Rui.,Zhang, Xu.,Zhang, Ge.,Tao, Tingting.,Yu HB.,...&Qin JH.(2017).Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice.Neurochemical Research,42(5),1478-1487. |
| MLA | Gu, Rui,et al."Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice".Neurochemical Research 42.5(2017):1478-1487. |
入库方式： OAI收割
来源：沈阳自动化研究所
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