Graphene Enhances Cellular Proliferation through Activating the Epidermal Growth Factor Receptor
文献类型：期刊论文
| 作者 | Liu, Wei; Sun, Cheng; Liao, Chunyang; Cui, Lin; Li, Haishan; Qu, Guangbo; Yu, Wenlian; Song, Naining; Cui, Yuan; Wang, Zheng |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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| 出版日期 | 2016-07-27 |
| 卷号 | 64期号:29页码:5909-5918 |
| 关键词 | graphene cell proliferation EGFR cytotoxicity biosafety |
| 英文摘要 | Graphene has promising applications in food packaging, water purification, and detective sensors for contamination monitoring. However, the biological effects of graphene are not fully understood. It is necessary to clarify the potential risks of graphene exposure to humans through diverse routes, such as foods. In the present study, graphene, as the model nanomaterial, was used to test its potential effects on the cell proliferation based on multiple representative cell lines, including HepG2, A549, MCF-7, and HeLa cells. Graphene was characterized by Raman spectroscopy, particle size analysis, atomic force microscopy, and transmission electron microscopy. The cellular responses to graphene exposure were evaluated using flow cytometry, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, and alamarBlue assays. Rat cerebral astrocyte cultures, as the non-cancer cells, were used to assess the potential cytotoxicity of graphene as well. The results showed that graphene stimulation enhanced cell proliferation in all tested cell cultures and the highest elevation in cell growth was up to 60%. A western blot assay showed that the expression of epidermal growth factor (EGF) was upregulated upon graphene treatment. The phosphorylation of EGF receptor (EGFR) and the downstream proteins, ShC and extracellular regulating kinase (ERK), were remarkably induced, indicating that the activation of the mitogen-activated protein kinase (MAPK)/ERK signaling pathway was triggered. The activation of PI3 kinase p85 and AKT showed that the PI3K/AKT signaling pathway was also involved in graphene-induced cell proliferation, causing the increase of cell ratios in the G2/M phase. No influences on cell apoptosis were observed in graphene-treated cells when compared to the negative controls, proving the low cytotoxicity of this emerging nanomaterial. The findings in this study revealed the potential cellular biological effect of graphene, which may give useful hints on its biosafety evaluation and the further exploration of the bioapplication. |
| 收录类别 | SCI |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/35863]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Liu, Wei,Sun, Cheng,Liao, Chunyang,et al. Graphene Enhances Cellular Proliferation through Activating the Epidermal Growth Factor Receptor[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2016,64(29):5909-5918. |
| APA | Liu, Wei.,Sun, Cheng.,Liao, Chunyang.,Cui, Lin.,Li, Haishan.,...&Zhou, Qunfang.(2016).Graphene Enhances Cellular Proliferation through Activating the Epidermal Growth Factor Receptor.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,64(29),5909-5918. |
| MLA | Liu, Wei,et al."Graphene Enhances Cellular Proliferation through Activating the Epidermal Growth Factor Receptor".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 64.29(2016):5909-5918. |
入库方式： OAI收割
来源：生态环境研究中心
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