Rapid Quantification and Quantitation of Alkaloids in Xinjiang Fritillaria by Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry
文献类型：期刊论文
| 作者 | Mohammat, A (Mohammat, Aziz); Yili, A (Yili, Abulimiti); Aisa, HA (Aisa, Haji Akber)
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| 刊名 | MOLECULES
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| 出版日期 | 2017 |
| 卷号 | 22期号:5 |
| 关键词 | UPLC-QTOF-MS Fritillariae qualitative and quantitative analysis alkaloids |
| 通讯作者 | Aisa, HA |
| 英文摘要 | The Fritillaria genus, including different kinds of medicinal and edible plants belonging to the Liliaceae family which have the function of treating and relieving a cough and eliminating phlegm, is widely planted in Xinjiang (China). There are few comprehensive studies reporting on the characterization of the chemical constituents of Fritillaria from Xinjiang, and to date, no work describing the quantitative differences between the components in Fritillaria from Xinjiang and related species. The purpose of this study was to develop qualitative and quantitative analytical methods by Ultra Performance Liquid Chromatography-Quadrupole Time-of-flight Mass Spectrometry (UPLC-QTOF-MS) for the rapid quantification and quantitation of alkaloids in wild and cultivated Xinjiang Fritillaria, which could be used in the quality control of medicine based on this natural herb. Using the UPLC-QTOF-MS method, the chemical constituents of Xinjiang Fritillaria were identified by fragmentation information and retention behavior, and were compared to reference standards. Furthermore, a quantitative comparision of four major alkaloids in wild and cultivated Xinjiang Fritillaria was conducted by determining the content of Sipeimine-3 beta-D-glucoside, Sipeimine, Peimisine, and Yibeinoside A, respectively. A total of 89 characteristic peaks, including more than 40 alkaloids, were identified in the chromatographic results of Fritillaria. Four main alkaloids were quantified by using a validated method based on UPLC-QTOF-MS. The relative contents of Sipeimine-3 beta-D-glucoside, Sipeimine, Peimisine, and Yibeinoside A varied from 0.0013%similar to 0.1357%, 0.0066%similar to 0.1218%, 0.0033%similar to 0.0437%, and 0.0019%similar to 0.1398%, respectively. A rough separation of wild and cultivated Fritillaria could be achieved by the cluster analysis method. |
| 收录类别 | SCI |
| WOS记录号 | WOS:000404522900042 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/4861]  |
| 专题 | 新疆理化技术研究所_中国科学院特殊环境功能材料与器件重点试验室 新疆理化技术研究所_资源化学研究室 |
| 作者单位 | 1.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China 2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Xinjiang Inst Food & Drug Control, Urumqi 830000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mohammat, A ,Yili, A ,Aisa, HA . Rapid Quantification and Quantitation of Alkaloids in Xinjiang Fritillaria by Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry[J]. MOLECULES,2017,22(5). |
| APA | Mohammat, A ,Yili, A ,&Aisa, HA .(2017).Rapid Quantification and Quantitation of Alkaloids in Xinjiang Fritillaria by Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry.MOLECULES,22(5). |
| MLA | Mohammat, A ,et al."Rapid Quantification and Quantitation of Alkaloids in Xinjiang Fritillaria by Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry".MOLECULES 22.5(2017). |
入库方式： OAI收割
来源：新疆理化技术研究所
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