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NIP1; 2 is a plasma membrane-localized transporter mediating aluminum uptake, translocation, and tolerance in Arabidopsis

文献类型：期刊论文

作者Jia, Xiaomin7; Liu, Jiping7; Kochian, Leon V.7,8; Huang, Yulan1,7; Hou, Siyu2,7; Lyi, Sangbom M.7; Li, Jianyong3,7; Zhou, Dangwei4,7; Li, Demou5,7; Li, Ruihong6,7
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版日期2017-05-09
卷号114期号:19页码:5047-5052
关键词aquaporin nodulin 26-like protein aluminum tolerance organic acid exudation malate
英文摘要Members of the aquaporin (AQP) family have been suggested to transport aluminum (Al) in plants; however, the Al form transported by AQPs and the roles of AQPs in Al tolerance remain elusive. Here we report that NIP1; 2, a plasma membrane-localized member of the Arabidopsis nodulin 26-like intrinsic protein (NIP) subfamily of the AQP family, facilitates Al-malate transport from the root cell wall into the root symplasm, with subsequent Al xylem loading and root-toshoot translocation, which are critical steps in an internal Al tolerance mechanism in Arabidopsis. We found that NIP1; 2 transcripts are expressed mainly in the root tips, and that this expression is enhanced by Al but not by other metal stresses. Mutations in NIP1; 2 lead to hyperaccumulation of toxic Al3+ in the root cell wall, inhibition of root-to-shoot Al translocation, and a significant reduction in Al tolerance. NIP1; 2 facilitates the transport of Al-malate, but not Al3+ ions, in both yeast and Arabidopsis. We demonstrate that the formation of the Al-malate complex in the root tip apoplast is a prerequisite for NIP1; 2-mediated Al removal from the root cell wall, and that this requires a functional root malate exudation system mediated by the Al-activated malate transporter, ALMT1. Taken together, these findings reveal a critical linkage between the previously identified Al exclusion mechanism based on root malate release and an internal Al tolerance mechanism identified here through the coordinated function of NIP1; 2 and ALMT1, which is required for Al removal from the root cell wall, root-to-shoot Al translocation, and overall Al tolerance in Arabidopsis.
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]MAJOR INTRINSIC PROTEINS ; TRANSITION ZONE ; MALATE TRANSPORTER ; INDUCED INHIBITION ; ACTIVATED CITRATE ; PLANT AQUAPORINS ; WHEAT ROOTS ; ACID SOILS ; RESISTANCE ; GENES
收录类别SCI
语种英语
WOS记录号WOS:000400818400059
源URL[http://ir.nwipb.ac.cn/handle/363003/7065]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
作者单位1.Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China
2.Shanxi Agr Univ, Sch Agr, Jinzhong 030801, Peoples R China
3.Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA
4.Chinese Acad Sci, Northwest Inst Plateau, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China
5.Southwest Univ, Biotechnol Res Ctr, Chongqing 400715, Peoples R China
6.Chinese Acad Sci, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
7.ARS, Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY 14853 USA
8.Univ Saskatchewan, Global Inst Food Secur, Saskatoon, SK S7N 4J8, Canada
推荐引用方式
GB/T 7714		 Jia, Xiaomin,Liu, Jiping,Kochian, Leon V.,et al. NIP1; 2 is a plasma membrane-localized transporter mediating aluminum uptake, translocation, and tolerance in Arabidopsis[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2017,114(19):5047-5052.
APA Jia, Xiaomin.,Liu, Jiping.,Kochian, Leon V..,Huang, Yulan.,Hou, Siyu.,...&Wang, Yuqi.(2017).NIP1; 2 is a plasma membrane-localized transporter mediating aluminum uptake, translocation, and tolerance in Arabidopsis.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,114(19),5047-5052.
MLA Jia, Xiaomin,et al."NIP1; 2 is a plasma membrane-localized transporter mediating aluminum uptake, translocation, and tolerance in Arabidopsis".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114.19(2017):5047-5052.

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来源：西北高原生物研究所

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