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Single-Cell Resolution of Uncultured Magnetotactic Bacteria via Fluorescence-Coupled Electron Microscopy

文献类型：期刊论文

作者Li, Jinhua1,2,3; Zhang, Heng1,2,3,4; Menguy, Nicolas3,5; Benzerara, Karim5; Wang, Fuxian1,2,3; Lin, Xiaoting1,4; Chen, Zhibao4; Pan, Yongxin1,3
刊名APPLIED AND ENVIRONMENTAL MICROBIOLOGY
出版日期2017-06-01
卷号83期号:12
关键词Biomineralization Fluorescence-coupled Electron Microscopy Fish Magnetotactic Bacteria Tem
ISSN号0099-2240
DOI10.1128/AEM.00409-17
文献子类Article
英文摘要Magnetotactic bacteria (MTB) form intracellular chain-assembled nanocrystals of magnetite or greigite termed magnetosomes. The characterization of magnetosome crystals requires electron microscopy due to their nanoscopic sizes. However, electron microscopy does not provide phylogenetic information for MTB. We have developed a strategy for the simultaneous and rapid phylogenetic and biomineralogical characterization of uncultured MTB at the single-cell level. It consists of four steps: (i) enrichment of MTB cells from an environmental sample, (ii) 16S rRNA gene sequencing of MTB, and (iii) fluorescence in situ hybridization analyses coordinated with (iv) transmission or scanning electron microscopy of the probe-hybridized cells. The application of this strategy identified a magnetotactic Gammaproteobacteria strain, SHHR-1, from brackish sediments collected from the Shihe River estuary in Qinhuangdao City, China. SHHR-1 magnetosomes are elongated prismatic magnetites which can be idealized as hexagonal prisms. Taxonomic groups of uncultured MTB were also identified in freshwater sediments from Lake Miyun in northern Beijing via this novel coordinated fluorescence and scanning electron microscopy method based on four group-specific rRNA-targeted probes. Our analyses revealed that major magnetotactic taxonomic groups can be accurately determined only with coordinated scanning electron microscopy observations on fluorescently labeled single cells due to limited group coverage and specificity for existing group-specific MTB fluorescence in situ hybridization (FISH) probes. Our reported strategy is simple and efficient, offers great promise toward investigating the diversity and biomineralization of MTB, and may also be applied to other functional groups of microorganisms.
WOS关键词IN-SITU HYBRIDIZATION ; CATALYZED REPORTER DEPOSITION ; MICROBIAL COMMUNITIES ; MAGNETOSOME FORMATION ; MAGNETIC-PROPERTIES ; PHYLUM NITROSPIRAE ; IDENTIFICATION ; BIOMINERALIZATION ; DIVERSITY ; PROBES
WOS研究方向Biotechnology & Applied Microbiology ; Microbiology
语种英语
WOS记录号WOS:000402386500015
出版者AMER SOC MICROBIOLOGY
资助机构National Natural Science Foundation of China(41522402 ; National Natural Science Foundation of China(41522402 ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; 41374004 ; 41374004 ; 41330104 ; 41330104 ; 41621004) ; 41621004) ; National Natural Science Foundation of China(41522402 ; National Natural Science Foundation of China(41522402 ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; 41374004 ; 41374004 ; 41330104 ; 41330104 ; 41621004) ; 41621004) ; National Natural Science Foundation of China(41522402 ; National Natural Science Foundation of China(41522402 ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; 41374004 ; 41374004 ; 41330104 ; 41330104 ; 41621004) ; 41621004) ; National Natural Science Foundation of China(41522402 ; National Natural Science Foundation of China(41522402 ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; 41374004 ; 41374004 ; 41330104 ; 41330104 ; 41621004) ; 41621004)
源URL[http://ir.iggcas.ac.cn/handle/132A11/52861]  
专题地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者Li, Jinhua
作者单位1.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Beijing, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Geol, Qingdao, Peoples R China
3.Chinese Acad Sci, France China Biomineralizat & Nanostruct Lab, Beijing, Peoples R China
4.Heilongjiang Bayi Agr Univ, Coll Life Sci & Technol, Daqing, Peoples R China
5.Sorbonne Univ, IMPMC, CNRS UMR 7590, MNHN,UPMC,IRD UMR 206, Paris, France
推荐引用方式
GB/T 7714		 Li, Jinhua,Zhang, Heng,Menguy, Nicolas,et al. Single-Cell Resolution of Uncultured Magnetotactic Bacteria via Fluorescence-Coupled Electron Microscopy[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2017,83(12).
APA Li, Jinhua.,Zhang, Heng.,Menguy, Nicolas.,Benzerara, Karim.,Wang, Fuxian.,...&Pan, Yongxin.(2017).Single-Cell Resolution of Uncultured Magnetotactic Bacteria via Fluorescence-Coupled Electron Microscopy.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,83(12).
MLA Li, Jinhua,et al."Single-Cell Resolution of Uncultured Magnetotactic Bacteria via Fluorescence-Coupled Electron Microscopy".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.12(2017).

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来源：地质与地球物理研究所

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