Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy
文献类型：期刊论文
| 作者 | Zhang, Weige; He, Wenhui; Xu, Hanying; Liu LQ(刘连庆) ; Ma EL(马恩龙); Li YC(李艳春); Chen, Jv; Liu, Yutong
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| 刊名 | PLOS ONE
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| 出版日期 | 2017 |
| 卷号 | 12期号:6页码:1-16 |
| ISSN号 | 1932-6203 |
| 产权排序 | 1 |
| 通讯作者 | Ma EL(马恩龙) |
| 中文摘要 | As an inhibitor of microtubule assembly, combretastatin A-4 (CA-4)-induced biological responses in tumor cells have been well known, but the corresponding changes in nano-biophysical properties were not investigated given the lack of an ideal tool. Using AFM technique, we investigated the alteration of nano-biophysical properties when CA-4-treated tumor cells underwent the different biological processes, including cell cycle arrest, apoptosis and autophagy. We found that CA-4-resistant cells were rougher with the presence of characteristic "ridges", indicating that the development of "ridge" structure may be a determinant of the sensitivity of cells to CA-4 compounds. CA-4 induced G2/M arrest and apoptosis in sensitive cells but triggered anti-apoptotic autophagy in resistant cells. CA-4 treatment caused an increase in stiffness in both sensitive and resistant cells. However, these cells exhibited different changes in cell surface roughness. CA-4 decreased Ra and Rq values in sensitive cells but increased these values in resistant cells. The reorganization of F-actin might contribute to the different changes of nano-biophysical properties in CA-4-sensitive and-resistant cells. Our results suggest that cellular nano-biophysical properties, such as "ridges", roughness and stiffness, could be applied as potential biomarkers for evaluating CA-4 compounds, and knowledge regarding how biological alterations cause changes in cellular nano-biophysical properties is helpful to develop a new high-resolution screening tool for anti-tumor agents. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | AUTOPHAGY ; APOPTOSIS ; MECHANICS ; TUBULIN ; ARREST ; AGENT ; A-4 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000404043100016 |
| 源URL | [http://ir.sia.cn/handle/173321/20804]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 作者单位 | 1.Department of Medicinal Chemistry, Shenyang Pharmaceutical University, Shenyang, China 2.Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, China 3.State Key Laboratory of Robotics, Shenyang Institute of Automation, China Academy of Sciences, Shenyang, China |
| 推荐引用方式 GB/T 7714 | Zhang, Weige,He, Wenhui,Xu, Hanying,et al. Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy[J]. PLOS ONE,2017,12(6):1-16. |
| APA | Zhang, Weige.,He, Wenhui.,Xu, Hanying.,Liu LQ.,Ma EL.,...&Liu, Yutong.(2017).Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy.PLOS ONE,12(6),1-16. |
| MLA | Zhang, Weige,et al."Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy".PLOS ONE 12.6(2017):1-16. |
入库方式： OAI收割
来源：沈阳自动化研究所
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