Determination of Cell Membrane Capacitance and Conductance via Optically Induced Electrokinetics
文献类型：期刊论文
| 作者 | Liang WF(梁文峰); Zhao YL(赵玉良); Li GB(李国宾); Li WJ(李文荣) ; Wang YC(王越超); Liu LQ(刘连庆)
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| 刊名 | BIOPHYSICAL JOURNAL
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| 出版日期 | 2017 |
| 卷号 | 113期号:7页码:1531-1539 |
| ISSN号 | 0006-3495 |
| 产权排序 | 1 |
| 通讯作者 | Liu LQ(刘连庆) ; Li WJ(李文荣) |
| 中文摘要 | Cell membrane capacitance and conductance are key pieces of intrinsic information correlated with the cellular dielectric parameters and morphology of the plasma membrane; these parameters have been used as electrophysiological biomarkers to characterize cellular phenotype and state, and they have many associated clinical applications. Here, we present our work on the non-invasive determination of cell membrane capacitance and conductance by an optically activated microfluidics chip. The model for determining the cell membrane capacitance and conductance was established by a single layer of the shellcore polarization model. Three-dimensional finite-element analyses of the positive and negative optically induced dielectrophoresis forces generated by the projected light arrays of spots were performed, thus providing a theoretical validation of the feasibility of this approach. Then, the crossover frequency spectra for four typical types of cells (Raji cells, MCF-7 cells, HEK293 cells, and K562 cells) were experimentally investigated by using a micro-vision based motion-tracking technique. The different responses of these cells to the positive and negative ODEP forces were studied under four different liquid conductivities by automatic observation and tracking of the cellular trajectory and texture during the cells' translation. The cell membrane capacitance and conductance were determined from the curve-fitted spectra, which were 11.1 +/- 0.9 mF/m(2) and 782 +/- 32 S/m(2), respectively, for Raji cells, 11.5 +/- 0.8 mF/m(2) and 114 +/- 28 S/m(2) for MCF-7 cells, 9.0 +/- 0.9 mF/m(2) and 187 +/- 22 S/m(2) for HEK293 cells, and 10.2 +/- 0.7 mF/m(2) and 879 +/- 24 S/m(2) for K562 cells. Furthermore, as an application of this technique, the membrane capacitances of MCF-7 cells treated with four different concentrations of drugs were acquired. This technique introduces a determination of cell membrane capacitance and conductance that yields statistically significant data while allowing information from individual cells to be obtained in a non-invasive manner. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biophysics |
| 研究领域[WOS] | Biophysics |
| 关键词[WOS] | ELECTRIC-FIELD ; CANCER-CELLS ; TUMOR-CELLS ; DIELECTROPHORESIS ; ELECTROROTATION ; MANIPULATION ; CYTOPLASM ; FREQUENCY ; INTERPLAY ; EXHIBIT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000412551200016 |
| 源URL | [http://ir.sia.cn/handle/173321/21107]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 作者单位 | 1.School of Mechanical Engineering, Shenyang Jianzhu University, Shenyang, China 2.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China 3.Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan 4.School of Control Engineering, Northeastern University at Qinhuangdao, Qinhuangdao, China 5.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong |
| 推荐引用方式 GB/T 7714 | Liang WF,Zhao YL,Li GB,et al. Determination of Cell Membrane Capacitance and Conductance via Optically Induced Electrokinetics[J]. BIOPHYSICAL JOURNAL,2017,113(7):1531-1539. |
| APA | Liang WF,Zhao YL,Li GB,Li WJ,Wang YC,&Liu LQ.(2017).Determination of Cell Membrane Capacitance and Conductance via Optically Induced Electrokinetics.BIOPHYSICAL JOURNAL,113(7),1531-1539. |
| MLA | Liang WF,et al."Determination of Cell Membrane Capacitance and Conductance via Optically Induced Electrokinetics".BIOPHYSICAL JOURNAL 113.7(2017):1531-1539. |
入库方式： OAI收割
来源：沈阳自动化研究所
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