Mask-free fabrication of a versatile microwell chip for multidimensional cellular analysis and drug screening
文献类型：期刊论文
| 作者 | Liu LQ(刘连庆) ; Yu HB(于海波); Yang WG(杨文广); Wei FN(魏发南); Li GX(李恭新); Wang YC(王越超)
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| 刊名 | Lab on a Chip
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| 出版日期 | 2017 |
| 卷号 | 17期号:24页码:4243-4252 |
| ISSN号 | 1473-0197 |
| 产权排序 | 1 |
| 通讯作者 | Yu HB(于海波) ; Liu LQ(刘连庆) |
| 中文摘要 | Cells are frequently studied because they are basic structural, functional, and biological units of living organisms. Extracting features from cellular behaviors can facilitate decision making in medical diagnoses and represents an important aspect in the development of biomedical engineering. Previous studies have just focused on either the individual cell or cell clusters separately, which leads to a great lack of information. Microwell technologies could address the challenges of in vitro cellular studies, from individual cell studies to 3D functional assays, by providing more information from smaller sample volumes and enabling the incorporation of low-cost high-throughput assays in the drug discovery process. To this end, the present study describes an easy-to-use method for fabricating a versatile microwell chip that utilizes a digital micro-mirror device printing system, and the chip can be employed in multidimensional cellular analysis, ranging from the single cell to the 3D spheroid level. The microwell manufacturing process, using a digital mask in place of a conventional physical mask, is based on shadowed light and is full of flexibility. Three different dimensions (single cell (1D), cell monolayer (2D) and cell spheroid (3D)) of one cell type can be formed using a microwell array and the analyses of biological characteristics are achieved separately. Single cells and cell clusters can be controlled via customized geometries of microfabricated selectively adhesive poly(ethylene glycol) diacrylate (PEGDA) wells. The effects of shape on cellular growth and hybrid tissue layers were investigated by peeling off the microwells. Furthermore, 3D multicellular spheroids were successfully established in a controllable and high-throughput format. Preclinical drug screening was investigated and distinct differences were observed in the tolerance response to drug testing between the 2D and 3D conditions. The study results further demonstrate that the high-density microwell chip is an easy-to-use multidimensional cellular analysis and rapid drug screening technique, and it could be easily adapted for a wide range of biological research applications. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics |
| 关键词[WOS] | CANCER-CELLS ; MULTICELLULAR SPHEROIDS ; SINGLE CELLS ; STEM-CELLS ; ARRAY ; MICROARRAYS ; CULTURE ; SIZE ; MECHANICS ; SCAFFOLDS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000417107500006 |
| 源URL | [http://ir.sia.cn/handle/173321/21393]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 作者单位 | 1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110000, P. R. China 2.University of Chinese Academy of Sciences, Beijing 100049, P. R. China 3.School of Mechanical Engineering and Automation, Fuzhou University, Fuzhou 350000, P. R. China |
| 推荐引用方式 GB/T 7714 | Liu LQ,Yu HB,Yang WG,et al. Mask-free fabrication of a versatile microwell chip for multidimensional cellular analysis and drug screening[J]. Lab on a Chip,2017,17(24):4243-4252. |
| APA | Liu LQ,Yu HB,Yang WG,Wei FN,Li GX,&Wang YC.(2017).Mask-free fabrication of a versatile microwell chip for multidimensional cellular analysis and drug screening.Lab on a Chip,17(24),4243-4252. |
| MLA | Liu LQ,et al."Mask-free fabrication of a versatile microwell chip for multidimensional cellular analysis and drug screening".Lab on a Chip 17.24(2017):4243-4252. |
入库方式： OAI收割
来源：沈阳自动化研究所
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