siRNA介导S100A4基因沉默对胃癌细胞中MMP-2和E-cadherin表达及细胞侵袭能力的影响
文献类型：期刊论文
| 作者 | 赵滢1; 张天彪3; 高立红1; 王楠1; 鲍晨辉1; 王文学2; 赵新刚 ; 赵明; 姚杰
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| 刊名 | 解剖科学进展
 |
| 出版日期 | 2018 |
| 卷号 | 24期号:4页码:364-367 |
| 关键词 | 胃癌 S100A4 基质金属蛋白酶2 E-钙黏素 基因沉默 |
| ISSN号 | 1006-2947 |
| 其他题名 | Effects of siRNA-mediated S100A4 silencing on MMP-2 and E-cadherin expression and invasive ability of gastric cancer cells |
| 产权排序 | 2 |
| 通讯作者 | 赵滢 |
| 中文摘要 | 目的 观察用RNA干扰技术沉默人胃癌细胞株MGC-803中钙结合蛋白S100A4的基因,对基质金属蛋白酶2(matrix metalloproteinase 2,MMP-2)和E-钙黏素(E-cadherin)表达变化,探讨S100A4对胃癌细胞侵袭能力的影响及作用机制。方法 采用RNA干扰技术将针对S100A4和非特异性阴性对照序列的si RNA转染至人胃癌MGC-803细胞,利用荧光实时定量RT-PCR和蛋白免疫印迹法(Western Blot)检测S100A4基因和蛋白的表达,筛选S100A4基因沉默组和阴性对照组细胞。采用Western Blot方法检测MMP-2和E-cadherin蛋白表达。利用Transwell Matrigel方法检测各实验组中MGC-803细胞体外侵袭能力。结果 成功建立S100A4基因沉默的人胃癌MGC-803细胞。S100A4基因沉默的胃癌MGC-803细胞中MMP-2蛋白表达降低,而E-cadherin蛋白表达明显上调;细胞体外侵袭能力亦显著下降。结论 S100A4能通过调控MMP-2和E-cadherin的表达影响胃癌细胞MGC-803的侵袭能力。 |
| 英文摘要 | Objective To explore the effects of siRNA-mediated S100A4 silencing on MMP-2 and E-cadherin expression and invasive ability of human gastric cancer cell lines MGC-803. Methods The S100A4-siRNA was transfected into human gastric cancer MGC-803 cells. The expressions of S100A4 mRNA and protein were detected by Real-time RT-PCR and Western blot to select S100A4 silencing and negative control cells. The levels of MMP-2 and Ecadherin proteins were determined by Western blot. The changes of invasive abilities were detected by Transwell chamber model. Results The human gastric cancer MGC-803 cells of S100A4 silencing were successfully established. Compared with the control group, the levels of S100A4 gene and protein were significantly decreased, the expression levels of MMP-2 protein decreased and the level of E-cadherin increased significantly，the invasive ability was reduced obviously In MGC- 803 cells of S100A silencing. Conclusion S100A4 could regulate the expression of MMP-2 and E-cadherin to affect the invasive abilities of human gastric cancer MGC-803 cells. |
| 语种 | 中文 |
| 源URL | [http://ir.sia.cn/handle/173321/22405]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 作者单位 | 1.中国医科大学附属盛京医院胃肠营养外科 2.机器人学国家重点实验室(中国科学院沈阳自动化研究所) 3.中国医科大学基础医学院生物化学与分子生物学教研室 |
| 推荐引用方式 GB/T 7714 | 赵滢,张天彪,高立红,等. siRNA介导S100A4基因沉默对胃癌细胞中MMP-2和E-cadherin表达及细胞侵袭能力的影响[J]. 解剖科学进展,2018,24(4):364-367. |
| APA | 赵滢.,张天彪.,高立红.,王楠.,鲍晨辉.,...&姚杰.(2018).siRNA介导S100A4基因沉默对胃癌细胞中MMP-2和E-cadherin表达及细胞侵袭能力的影响.解剖科学进展,24(4),364-367. |
| MLA | 赵滢,et al."siRNA介导S100A4基因沉默对胃癌细胞中MMP-2和E-cadherin表达及细胞侵袭能力的影响".解剖科学进展 24.4(2018):364-367. |
入库方式： OAI收割
来源：沈阳自动化研究所
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