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RTF: a rapid and versatile tissue optical clearing method

文献类型：期刊论文

作者Yu, Tingting3,4; Zhu, Jingtan3,4; Li, Yusha3,4; Ma, Yilin3,4; Wang, Jianru3,4; Cheng, Xinran5; Jin, Sen2; Sun, Qingtao3,4; Li, Xiangning3,4; Gong, Hui3,4
刊名SCIENTIFIC REPORTS
出版日期2018-01-31
卷号8
ISSN号2045-2322
DOI10.1038/s41598-018-20306-3
文献子类Article
英文摘要Tissue optical clearing enables imaging deeper in large volumes with high-resolution. Clear(T2) is a relatively rapid clearing method with no use of solvents or detergents, hence poses great advantage on preservation of diverse fluorescent labels. However, this method suffers from insufficient tissue transparency, especially for adult mouse brain blocks. In this work, we develop a rapid and versatile clearing method based on Clear(T2), termed RTF (Rapid clearing method based on Triethanolamine and Formamide), aiming for better clearing capability. The results show that RTF can not only efficiently clear embryos, neonatal brains and adult brain blocks, but also preserve fluorescent signal of both endogenous fluorescent proteins and lipophilic dyes, and be compatible with virus labeling and immunostaining. With the good transparency and versatile compatibility, RTF allows visualization and tracing of fluorescent labeling cells and neuronal axons combined with different imaging techniques, showing potentials in facilitating observation of morphological architecture and visualization of neuronal networks.
WOS关键词SINGLE-CELL RESOLUTION ; WHOLE-BODY ; SCATTERING PROPERTIES ; MOUSE-BRAIN ; SYSTEMS ; AGENT ; RECONSTRUCTION ; PROTOCOLS ; ORGANS
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000423663100043
出版者NATURE PUBLISHING GROUP
资助机构National Natural Science Foundation of China(31571002 ; National Natural Science Foundation of China(31571002 ; Science Fund for Creative Research Group of China(61721092) ; Science Fund for Creative Research Group of China(61721092) ; China Postdoctoral Science Foundation(2017M612463) ; China Postdoctoral Science Foundation(2017M612463) ; Director Fund of Wuhan National Laboratory for Optoelectronics ; Director Fund of Wuhan National Laboratory for Optoelectronics ; 81701354) ; 81701354) ; National Natural Science Foundation of China(31571002 ; National Natural Science Foundation of China(31571002 ; Science Fund for Creative Research Group of China(61721092) ; Science Fund for Creative Research Group of China(61721092) ; China Postdoctoral Science Foundation(2017M612463) ; China Postdoctoral Science Foundation(2017M612463) ; Director Fund of Wuhan National Laboratory for Optoelectronics ; Director Fund of Wuhan National Laboratory for Optoelectronics ; 81701354) ; 81701354) ; National Natural Science Foundation of China(31571002 ; National Natural Science Foundation of China(31571002 ; Science Fund for Creative Research Group of China(61721092) ; Science Fund for Creative Research Group of China(61721092) ; China Postdoctoral Science Foundation(2017M612463) ; China Postdoctoral Science Foundation(2017M612463) ; Director Fund of Wuhan National Laboratory for Optoelectronics ; Director Fund of Wuhan National Laboratory for Optoelectronics ; 81701354) ; 81701354) ; National Natural Science Foundation of China(31571002 ; National Natural Science Foundation of China(31571002 ; Science Fund for Creative Research Group of China(61721092) ; Science Fund for Creative Research Group of China(61721092) ; China Postdoctoral Science Foundation(2017M612463) ; China Postdoctoral Science Foundation(2017M612463) ; Director Fund of Wuhan National Laboratory for Optoelectronics ; Director Fund of Wuhan National Laboratory for Optoelectronics ; 81701354) ; 81701354)
源URL[http://ir.wipm.ac.cn/handle/112942/12838]  
专题中国科学院武汉物理与数学研究所
通讯作者Zhu, Dan
作者单位1.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai 200031, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Phys & Math, Ctr Brain Sci, Wuhan 430071, Hubei, Peoples R China
3.Huazhong Univ Sci & Technol, Britton Chance Ctr Biomed Photon, Wuhan Natl Lab Optoelect, Wuhan 430074, Peoples R China
4.Huazhong Univ Sci & Technol, Collaborat Innovat Ctr Biomed Engn, Sch Engn Sci, MoE Key Lab Biomed Photon, Wuhan 430074, Hubei, Peoples R China
5.Northwest A&F Univ, Coll Vet Med, Yangling 712100, Peoples R China
推荐引用方式
GB/T 7714		 Yu, Tingting,Zhu, Jingtan,Li, Yusha,et al. RTF: a rapid and versatile tissue optical clearing method[J]. SCIENTIFIC REPORTS,2018,8.
APA Yu, Tingting.,Zhu, Jingtan.,Li, Yusha.,Ma, Yilin.,Wang, Jianru.,...&Zhu, Dan.(2018).RTF: a rapid and versatile tissue optical clearing method.SCIENTIFIC REPORTS,8.
MLA Yu, Tingting,et al."RTF: a rapid and versatile tissue optical clearing method".SCIENTIFIC REPORTS 8(2018).

入库方式： OAI收割

来源：武汉物理与数学研究所

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