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Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae

文献类型：期刊论文

作者Xu, Mingyue2; Yang, Xizhe2; Yang, Xiangna2; Liu, Jingying1,2; Ma, PD (reprint author), Northwest A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China.; Ma, Pengda2; Xue, Mengmeng2; Liu, Jie2
刊名MOLECULAR BIOLOGY REPORTS
出版日期2017
卷号44期号:1页码:5-9
ISSN号0301-4851
关键词Stress Associated Protein Salinity Leymus Chinensis Saccharomyces Cerevisiae
DOI10.1007/s11033-016-4091-y
文献子类Article
英文摘要A number of members of the SAP ("stress-associated protein") gene family have been implicated in the plant stress response. Here, a SAP gene has been isolated using PCR RACE from the perennial grass Leymus chinensis, a species which has reputation for ecological adaptability. The 17.6 kDa LcSAP product comprised 161 residues, including both an A20 domain and an AN1 domain, a feature of type I SAPs. Using a semi-quantitative RT-PCR assay to profile its transcription, it was shown that LcSAP was more strongly transcribed in the leaf than in the root under control conditions. The level of LcSAP transcription began to rise 6 h after the plant's exposure to 400 mM NaCl, and the abundance of transcript remained stable for at least 24 h. Exposing the plant to 100 mM Na2CO3 also induced LcSAP transcription, but the abundance of SAP transcript faded after 6 h. When LcSAP was introduced into yeast cells, the transgenic cells grew better than wild type ones when the medium contained 1.4 M NaCl. The ability of LcSAP to respond to salinity stress in yeast suggests that it also makes a contribution to the stress tolerance shown by L. chinensis.
学科主题Biochemistry & Molecular Biology
URL标识查看原文
出版地DORDRECHT
语种英语
出版者SPRINGER
WOS记录号WOS:000394349600002
资助机构Chinese Universities Scientific Fund [2452016006, 2014YB044]; Specialized Research Fund for the Doctoral Program of Higher Education [20130204120018, 20130204120038]; Natural Science Basic Research Plan in Shaanxi Province of China [2013JQ3006]; National Natural Science Foundation of China [31401839, 31670295]; Institute of Soil and Water Conservation, NWAFU [A314021402-1522] ; Chinese Universities Scientific Fund [2452016006, 2014YB044]; Specialized Research Fund for the Doctoral Program of Higher Education [20130204120018, 20130204120038]; Natural Science Basic Research Plan in Shaanxi Province of China [2013JQ3006]; National Natural Science Foundation of China [31401839, 31670295]; Institute of Soil and Water Conservation, NWAFU [A314021402-1522] ; Chinese Universities Scientific Fund [2452016006, 2014YB044]; Specialized Research Fund for the Doctoral Program of Higher Education [20130204120018, 20130204120038]; Natural Science Basic Research Plan in Shaanxi Province of China [2013JQ3006]; National Natural Science Foundation of China [31401839, 31670295]; Institute of Soil and Water Conservation, NWAFU [A314021402-1522] ; Chinese Universities Scientific Fund [2452016006, 2014YB044]; Specialized Research Fund for the Doctoral Program of Higher Education [20130204120018, 20130204120038]; Natural Science Basic Research Plan in Shaanxi Province of China [2013JQ3006]; National Natural Science Foundation of China [31401839, 31670295]; Institute of Soil and Water Conservation, NWAFU [A314021402-1522]
源URL[http://ir.iswc.ac.cn/handle/361005/8038]  
专题水保所科研产出--SCI_2017--SCI
通讯作者Ma, PD (reprint author), Northwest A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China.
作者单位1.Northwest A&F Univ, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Peoples R China
2.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Mingyue,Yang, Xizhe,Yang, Xiangna,et al. Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae[J]. MOLECULAR BIOLOGY REPORTS,2017,44(1):5-9.
APA Xu, Mingyue.,Yang, Xizhe.,Yang, Xiangna.,Liu, Jingying.,Ma, PD .,...&Liu, Jie.(2017).Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae.MOLECULAR BIOLOGY REPORTS,44(1),5-9.
MLA Xu, Mingyue,et al."Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae".MOLECULAR BIOLOGY REPORTS 44.1(2017):5-9.

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