Comparative Genomics of Degradative Novosphingobium Strains With Special Reference to Microcystin-Degrading Novosphingobium sp THN1
文献类型：期刊论文
| 作者 | Zhao, Jindong1,3; Li, Tao3; Li, Renhui3; Shen, Mengyuan2,3; Li, Qi3; Bai, Peng2,3; Li, Jionghui2,3; Wang, Chang2,3; Wang, Juanping2,3 |
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2018-09-25 |
| 卷号 | 9页码:17 |
| 关键词 | Novosphingobium degradation comparative genomics genomic variability metabolic profile |
| ISSN号 | 1664-302X |
| DOI | 10.3389/fmicb.2018.02238 |
| 英文摘要 | Bacteria in genus Novosphingobium associated with biodegradation of substrates are prevalent in environments such as lakes, soil, sea, wood and sediments. To better understand the characteristics linked to their wide distribution and metabolic versatility, we report the whole genome sequence of Novosphingobium sp. THN1, a microcystin-degrading strain previously isolated by Jiang et al. (2011) from cyanobacteria-blooming water samples from Lake Taihu, China. We performed a genomic comparison analysis of Novosphingobium sp. THN1 with 21 other degradative Novosphingobium strains downloaded from GenBank. Phylogenetic trees were constructed using 16S rRNA genes, core genes, protein-coding sequences, and average nucleotide identity of whole genomes. Orthologous protein analysis showed that the 22 genomes contained 674 core genes and each strain contained a high proportion of distributed genes that are shared by a subset of strains. Inspection of their genomic plasticity revealed a high number of insertion sequence elements and genomic islands that were distributed on both chromosomes and plasmids. We also compared the predicted functional profiles of the Novosphingobium protein-coding genes. The flexible genes and all protein-coding genes produced the same heatmap clusters. The COG annotations were used to generate a dendrogram correlated with the compounds degraded. Furthermore, the metabolic profiles predicted from KEGG pathways showed that the majority of genes involved in central carbon metabolism, nitrogen, phosphate, sulfate metabolism, energy metabolism and cell mobility (above 62.5%) are located on chromosomes. Whereas, a great many of genes involved in degradation pathways (21-50%) are located on plasmids. The abundance and distribution of aromatics-degradative mono- and dioxygenases varied among 22 Novosphingoibum strains. Comparative analysis of the microcystin-degrading mlr gene cluster provided evidence for horizontal acquisition of this cluster. The Novosphingobium sp. THN1 genome sequence contained all the functional genes crucial for microcystin degradation and the mir gene cluster shared high sequence similarity (>= 85%) with the sequences of other microcystin-degrading genera isolated from cyanobacteria-blooming water. Our results indicate that Novosphingobium species have high genomic and functional plasticity, rearranging their genomes according to environment variations and shaping their metabolic profiles by the substrates they are exposed to, to better adapt to their environments. |
| 资助项目 | State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ08] ; Chinese Academy of Sciences[QYZDY-SSW-SMC004] ; Chinese Academy of Sciences[KFZD-SW-219] ; Science and Technology Basic Resources Investigation Program of China[2017FY100300] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000445584000001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/5719]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Li, Tao; Li, Renhui |
| 作者单位 | 1.Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Genet Engn, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Jindong,Li, Tao,Li, Renhui,et al. Comparative Genomics of Degradative Novosphingobium Strains With Special Reference to Microcystin-Degrading Novosphingobium sp THN1[J]. FRONTIERS IN MICROBIOLOGY,2018,9:17. |
| APA | Zhao, Jindong.,Li, Tao.,Li, Renhui.,Shen, Mengyuan.,Li, Qi.,...&Wang, Juanping.(2018).Comparative Genomics of Degradative Novosphingobium Strains With Special Reference to Microcystin-Degrading Novosphingobium sp THN1.FRONTIERS IN MICROBIOLOGY,9,17. |
| MLA | Zhao, Jindong,et al."Comparative Genomics of Degradative Novosphingobium Strains With Special Reference to Microcystin-Degrading Novosphingobium sp THN1".FRONTIERS IN MICROBIOLOGY 9(2018):17. |
入库方式： OAI收割
来源：武汉植物园
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