The destruction box is involved in the degradation of the nte family proteins by the proteasome
文献类型：期刊论文
| 作者 | Huang, Fei-Fei1; Chang, Ping-An1; Sun, Lan-Xi1; Qin, Wen-Zhen1; Han, Li-Ping1; Chen, Rui2 |
| 刊名 | Molecular biology reports
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 43期号:11页码:1285-1292 |
| 关键词 | Neuropathy target esterase Destruction box Ubiquitin-proteasome pathway Endoplasmic reticulum Cell growth |
| ISSN号 | 0301-4851 |
| DOI | 10.1007/s11033-016-4063-2 |
| 通讯作者 | Chang, ping-an(changpingan@aliyun.com) |
| 英文摘要 | Neuropathy target esterase (nte) and nte-related esterase (nre) are endoplasmic reticulum (er) membrane-anchored proteins belonging to the nte protein family. nte and nre are degraded by macroautophagy and by the ubiquitin-proteasome pathway. however, the regulation of nte and nre by proteasome has not been well understood. western blotting showed that the deletion of the regulatory region of nte and nre led to protein accumulation compared with that of the corresponding wild-type proteins. further, deletion and site-directed mutagenesis experiments demonstrated that the destruction (d) box was required for the proteasomal degradation of nte and nre. however, unlike the deletion of the regulatory region, the deletion of the d box did not affect the subcellular localisation of nte or nre or disrupt the er. moreover, the deletion of the d box or the regulatory region of nte has similar inhibitory effects on cell growth, which are greater than those produced by the full-length nte. here, for the first time, we show that the d box is involved in the regulation of nte family proteins by the proteasome but not in their subcellular localisation. in addition, these results suggest that the nte overexpression-mediated inhibition of cell growth is related to active protein levels but not to its er disruption effect. |
| WOS关键词 | NEUROPATHY TARGET ESTERASE ; CELL-PROLIFERATION ; MUTATIONS ; IDENTIFICATION ; EXPRESSION ; PATHWAY ; PNPLA6 ; LYSOPHOSPHOLIPASE ; NEURODEGENERATION ; FAILURE |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000387671100011 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176628 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Chang, Ping-An |
| 作者单位 | 1.Chongqing Univ Posts & Telecommun, Coll Bioinformat, Key Lab Mol Biol, Chongqing 400065, Peoples R China 2.Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Fei-Fei,Chang, Ping-An,Sun, Lan-Xi,et al. The destruction box is involved in the degradation of the nte family proteins by the proteasome[J]. Molecular biology reports,2016,43(11):1285-1292. |
| APA | Huang, Fei-Fei,Chang, Ping-An,Sun, Lan-Xi,Qin, Wen-Zhen,Han, Li-Ping,&Chen, Rui.(2016).The destruction box is involved in the degradation of the nte family proteins by the proteasome.Molecular biology reports,43(11),1285-1292. |
| MLA | Huang, Fei-Fei,et al."The destruction box is involved in the degradation of the nte family proteins by the proteasome".Molecular biology reports 43.11(2016):1285-1292. |
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来源：高能物理研究所
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