Interaction of gold and silver nanoparticles with human plasma: analysis of protein corona reveals specific binding patterns
文献类型：期刊论文
| 作者 | Lai, Wenjia1; Wang, Qingsong2; Li, Lumeng1; Hu, Zhiyuan1; Chen, Jiankui3; Fang, Qiaojun1,4 |
| 刊名 | Colloids and surfaces b-biointerfaces
 |
| 出版日期 | 2017-04-01 |
| 卷号 | 152页码:317-325 |
| 关键词 | Silver nanoparticle Gold nanoparticle Human plasma Corona Surface properties |
| ISSN号 | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2017.01.037 |
| 通讯作者 | Chen, jiankui(chenjiankui@hotmail.com) ; Fang, qiaojun(fangqj@nanoctr.cn) |
| 英文摘要 | Determining how nanomaterials interact with plasma will assist in understanding their effects on the biological system. this work presents a systematic study of the protein corona formed from human plasma on 20 nm silver and gold nanoparticles with three different surface modifications, including positive and negative surface charges. the results show that all nanoparticles, even those with positive surface modifications, acquire negative charges after interacting with plasma. approximately 300 proteins are identified on the coronas, while 99 are commonly found on each nanomaterial. the 20 most abundant proteins account for over 80% of the total proteins abundance. remarkably, the surface charge and core of the nanoparticles, as well as the isoelectric point of the plasma proteins, are found to play significant roles in determining the nanoparticle coronas. albumin and globulins are present at levels of less than 2% on these nanoparticle coronas. fibrinogen, which presents in the plasma but not in the serum, preferably binds to negatively charged gold nanoparticles. these observations demonstrate the specific plasma protein binding pattern of silver and gold nanoparticles, as well as the importance of the surface charge and core in determining the protein corona compositions. the potential downstream biological impacts of the corona proteins were also investigated. (c) 2017 elsevier b.v. all rights reserved. |
| WOS关键词 | COMPLEMENT ACTIVATION ; SERUM-ALBUMIN ; GENE ONTOLOGY ; SIZE ; SURFACES ; ADSORPTION ; FIBRINOGEN ; INTERFACE ; PROVIDE ; HARD |
| WOS研究方向 | Biophysics ; Chemistry ; Materials Science |
| WOS类目 | Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials |
| 语种 | 英语 |
| WOS记录号 | WOS:000398014100038 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176660 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Chen, Jiankui; Fang, Qiaojun |
| 作者单位 | 1.Chinese Acad Sci, Natl Ctr Nanosci & Technol, Ctr Excellence Nanosci, Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 2.Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China 3.Acad Mil Med Sci, Clin Lab, Affiliated Hosp, Beijing 100071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lai, Wenjia,Wang, Qingsong,Li, Lumeng,et al. Interaction of gold and silver nanoparticles with human plasma: analysis of protein corona reveals specific binding patterns[J]. Colloids and surfaces b-biointerfaces,2017,152:317-325. |
| APA | Lai, Wenjia,Wang, Qingsong,Li, Lumeng,Hu, Zhiyuan,Chen, Jiankui,&Fang, Qiaojun.(2017).Interaction of gold and silver nanoparticles with human plasma: analysis of protein corona reveals specific binding patterns.Colloids and surfaces b-biointerfaces,152,317-325. |
| MLA | Lai, Wenjia,et al."Interaction of gold and silver nanoparticles with human plasma: analysis of protein corona reveals specific binding patterns".Colloids and surfaces b-biointerfaces 152(2017):317-325. |
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